Using the flaxseed as the research object,separation,quantification,identification and analysis of the hydrolysates had been performed by reverse-liquid-chromatography and high performance liquid chromatography tandem mass spectrometry after extraction the lignan macromolecule from the flaxseed by 60% ethonal and lignin degradation enzyme hydrolysis. According to the highest yield of CaAG,HMGA + SDG,SDG + FeA,SDG + HMGA +CouA,SDG + 2HMGA + EtOH,HDG + HMGA of lignan macromolecule hydrolysates at the concentration of 0u /L,0. 005 u /L,0. 01 u /L,0. 015 u /L,0. 02 u /L,0. 025 u /L,0. 03 u /L of enzyme hydrolysis,0. 025 u /L lignin degradation enzyme was selected as the optimal concentration. The main enzyme hydrolysates were CaAG and SDG + HMGA+ CouA with the content of 7256 mg /kg and 2574 mg /kg. Based on the m /z-ratios and MS /MS data,four monomers including CaAG,CouAG,HDG,SDG among fourteen fractions of enzyme hydrolysates were detected,and ion fragment of SDG + HMGA( m /z = 685. 2[M-H+]-) and SDG( m/z =829.1[M-H+]-) were detected by MS/MS.