This work attempts to examine biogenic amine of Klebsiella sp.,Enterobacter cloacae strain,Pantoea agglomerans strain by high performance liquid chromatography. The screened strains were isolated from Xinjiang Smoked horse intestine samples. Quorum sensing is a cell-to-cell signaling system that functions by means of diffusible autoinducer( AI) signal molecules that allow the regulation of cellular processes in a manner that depends on the density of the microbial population. QS is a common development group behavior,which bacteria use signaling molecules produced by monitoring their own population density changes,regulation of gene expression,collaborative adapt to the environment. The parallel line experiment of Agrobacterium tumefaciens A136 and Agrobacterium tumefaciens KYC55,the results show that the biogenic amines strains can occurred blue change by X-gal. According to autoinducer activity test found that if the β-galactosidase activity of quorum sensing strains is higher,the more the bacteria secrete AHLs.AHLs were different secreted in different period of quorum sensing bacterial. Analysis of AHLs types of these strains by HPLC. Klebsiella sp. and Enterobacter cloacae strain can secrete signaling moleculesC4-HSL,C6-HSL,C8-HSL. Analysis of quorum sensing of biogenic amine strains,in order to establish basic theory about biogenic amine. It provides a good theoretical basis for the industrial production of fermented meat and security.