In this paper,a process for preparation of bagasse hydrolysate and production of succinic acid from bagasse by Actinobacillus succinogenes was studied.Bagasse pre-treated with 1% NaOH was degraded by complex enzymes comprising cellulase,xylanase,β-glucanase and pectinase.The reducing sugar concentration could be 65.6 g /L with a yield of 90% under the condition of 75 G /L substrate concentration.Using 55 g /L of bagasse hydrolysate as carbon source,fermentation was performed in a 3L stirred bioreactor,and 39.9 g /L succinic acid concentration was achieved with a succinic acid yield of 0.46 g /g(bagasse) at 29 h.The results showed the possibility of succinic acid production from bagasse.