Food and Fermentation Industries >

2014 , Vol. 40 >Issue 09: 23 - 28

DOI: https://doi.org/10.13995/j.cnki.11-1802/ts.2014.09.025

Food and Fermentation Industries

Synthesis of S-adenosylmethionine from adenine in constructing engineered strain and the screening of high yield strain

  • ZHENG Ji-rui ,
  • CHEN Li-fen ,
  • YANG Shan-yan ,
  • ZHU Jia-rong
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Online published: 2014-09-25

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Abstract

In this paper,Saccharomyces cerevisiae genome was used as a template,and the key enzyme gene sam2 was cloned into multiple cloning sites of the integrated plasmid pCB1004-Pgpd by PCR to obtain the recombinant plasmid for synthesis of SAM.The recombinant plasmid was transformed into Actinomucor elegans protoplast under the mediation of PEG-CaCl2 to get SAM productive engineering strain.With the adenine as precursor,the SAM production of screened strain increased from 0.40 g /L to 1.00 g /L as compared to the original strain,which increased by1.5 times.

Key words： S-adenosylmethionine; Actinomucor elegans; sam 2; adenine

Cite this article

ZHENG Ji-rui , CHEN Li-fen , YANG Shan-yan , ZHU Jia-rong . Synthesis of S-adenosylmethionine from adenine in constructing engineered strain and the screening of high yield strain[J]. Food and Fermentation Industries, 2014 , 40(09) : 23 -28 . DOI: 10.13995/j.cnki.11-1802/ts.2014.09.025

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