The effects of dissolved oxygen,shearing force and other parameters on the production of Lipstatin in shaking flasks and the scale-up fermentation,as well as continuous feeding process in 10 L fermenter were studied.The stirring process,ventilation and feeding process of 10 L fermenter were optimized to improve the dissolved oxygen level.Results showed that the potency of Lipstatin in 10 L fermenter reached 6 446 μg /mL,which was 40% higher than that in shaking flask fermentation,while fermentation time was 1day shorter.