The material was the aralosides extracted from Aralia elata(Miq) Seem which is planted in Benxi city Liaoning province.The main physical and chemical properties of the aralosides were analyzed after hydrolyzation with β-glucosidase in this study.The results indicated that the viscidity of the aralosides was decreased after hydrolyzation withβ-glucosidase,and the color of the araloside was changed from brown to buff,the solubility was improved,the volatilization was boosted,the stimulating odor was stronger,and the sweet taste was reduced.The proportions of quantity and oleanolic acid content in the six fractions separated by silica gel column were changed and the content of oleanolic acid in the aralosides rose from 33.5% to 37.2%.The capability of eliminating hydroxyl and superoxide anions free radical was positively related to aralosides concentration.The capability of eliminating free radical was improved obviously after hydrolyzation by β-glucosidase.The figure of ultraviolet absorption indicated that the quantity of the absorbing peaks reduced obviously,the absorption band was close to the shorter wavelength,and the biggest absorbing peak was changed from 299 ~ 236 nm after hydrolyzation by β-glucosidase.The figures of infrared absorption indicate that the functional groups such as aliphatic primary amines,secondary aliphatic alcohols,aliphatic carboxylic acids and unsaturated fatty acid appeared after hydrolyzation by β-glucosidase.The molecules of β-D galactose,phenyl glucoside,benzoic acid and long carbon chain emerged.These changes proved that the glucose molecule was released from the aralosides after hydrolyzation by the β-glucosidase,the molecules of the aralosides became smaller,the mount of monomers reduced,the main physical and chemical properties were changed,and the capability of eliminating free radical was improved obviously.All of these results gave a reference for the improvement of the physiological activities.
YAN Ting-Cai
,
ZHANG Xuan
,
SI qin
,
Meng XIAN-Jun
. Effect of hydrolyzing with β-glucosidase on the physical and chemical properties of aralosides[J]. Food and Fermentation Industries, 2014
, 40(09)
: 72
-77
.
DOI: 10.13995/j.cnki.11-1802/ts.2014.09.049