Acaudinaleucoproctaas was used in the study as raw material and the conditions of its extracting were optimized through single factor experiment and response surface methodology(RSM).The optimal conditions of Acaudinaleucoprocta collagen extraction were obtained based on the analysis of significant factors and their interactions.The results are as following:extraction temperature 80℃,water /solid ratio 13 /1,PH 7.6,extraction time 21 h.The extraction rate was 36.68%,and is fit with the prediction model.Water-extraction method did not only produce pure collagen,but also had no heavy metal residues.The study provided a reference for the development of Acaudinaleucoprocta.