The effects of oxidation stimulation and hypertonic stimulation on the production of GSH by waste beer yeast were studied in this research.These two kinds of stimulations were proved to effectively increase the production of GSH.In the oxidation stimulation,when 0.012 g /L of KMnO4 was added after fermentation for 21 hr,the production of GSH was 512 mg /L,which was increased 20% compared to that of control.When 30 mmol /L of H2O2 was added after fermentation for 12 h,the yield of GSH was highest.15 g /L KCl was added after 15 h of fermentation to get a higher production of GSH.The combination of these two kinds of stimulations showed no superimposed effect and less effective than using either of them alone.