By a screening method with high concentration of D-glucose in the medium,99 osmophilic yeasts were isolated from crude honeycomb and honey.Then,40 strains isolated from different samples were fermented in flaskshaking to study the variety of polyol in the fermentation broth by TLC(Thin Layer Chromatography) analysis methods.An effective method was established in this study to separate D-glucose and polyol.The qualitative detection of D-arabitol and byproducts was performed by TLC using a developing solvent system of normal ethyl acetate /pyridine /glacial acetic acid /water(16∶ 10∶ 2∶ 3) on microcrystalline cellulose plate.Experimental results showed that most of these yeasts were D-arabitol-producing and glycerol-producing strains.According to the observation and analyses of morphological,physiological and biochemical characteristics,ITS sequences and the established phylogenetic tree,the three different colonies were identified as Lodderomyces elongisporus,Zygosaccharomyces rouxii and Starmerella sp..A high-performing strain was isolated by screening tests,and the yield of D-arabitol was 61.33 ± 0.49 g /L,which detected by gas chromatography.The above results laid a foundation for further study on D-arabitol production by yeast.