Corynebacterium glutamicum was cultivated in two parallel 10 L fermenters under 5.0 m3/h and 3.5m3/h ventilation,respectively.The fermentation conditions of batch cultivation were investigated systematically.The experiment result showed that pH value decreased sharply when the ventilation was 5.0 m3/h.pH value was higher under 5.0 m3/h of ventilation than that under 3.5 m3/h at the end of the fermentation.High concentration of dissolved oxygen fermentation anaphase in fermentation liquid had a significant influence on DO.The L-tryptophan yield and the weight of dried cells reached 43.3 g /L and 18.3 g /L while the ventilation was 5.0 m3/h.Fermentation residual sugar did not change significantly.