Two water-souble polysaccharides,named LFI and LFII,were isolated from Lentinus edodes by 0. 9%Na Cl and hot water extraction,respectively. Three resins named AB-8,DA201 and JK008 were used to remove the pigments from LFI and LFII,wherein AB-8 was more suitable for removing the pigments with removal rate of 72% and53% from LFI and LFII respectively. The Mw of LFI and LFII was 1. 350 × 106 Da and 2. 771 × 106 Da,the Mn of LFI and LFII was 1. 337 × 106 Da and 2. 693 × 106 Da. IR,13C-NMR and1H-NMR studies indicated that LFI consisted of α-1Man,β-1Glc,α-1Gal,β-1,3-Glc-,α-1,6-Glc-,α-1,4-Glc- and α-1,4,6-Man6- residues,while LFII was found to contain α-1Xyl,α-1Gal,β-1Glc,β-1,3-Glc-,β-1,6-Glc-,α-1,6-Man-,β-1,3,6-Glc,α-1,4,6-Man- and α-1,2,6-Gal- residues.
ZHANG Hong-tao
,
ZHAO Xiao-liang
,
FAN Zun-xiao
,
LUO Cheng
,
ZHU Li
,
MAO Jiang-chuan
,
ZHAN Xiao-bei
,
MAO Yan-qing
. Isolation and refine of water-soluable polysaccharides LFI and LFII from Lentinus edudos body and structure characterization[J]. Food and Fermentation Industries, 2014
, 40(11)
: 1
-7
.
DOI: 10.13995/j.cnki.11-1802/ts.201411001
