Milk protein is the most important nutrients at the initial stage of the life. Using proteomics technology can be intuitive and comprehensive to study the composition of milk protein and regulation on controlling the life. It also can help to be more comprehensive and in-depth to illustrate the information of milk protein expression. This paper describes the application of proteomics in human milk and milk protein research progress,the research of milk casein Proteomics,whey proteomics,milk fat globule membrane proteomics,lactoferrin proteomics in order to find out the theoretical basis for clinical diagnosis of the disease and treatment as well as dairy processing storage conditions and the development of new infant foods.