A novel method for determination of Levofloxacin residues in chicken meat was developed based on charge transfer spectrophotometry. Levofloxacin and alizarin were reacted in mixed solution of water and ethanol. The complex compound had molar absorption of = 6. 47 × 103 L /( mol · cm) at 532 nm. The medical concentration obeyed the Beer 's law at 1 ~ 50 mg / L. The recovery was in the range of 92. 0% ~ 96. 8%,the RSDs of this method were less than 5. 6%,the detection limits was 1. 0 mg / kg( S / N = 3). The mechanism of the reaction was investigated by infrared spectroscopy.