Preservatives,saccharides,sugar,alcohol or polyols,salts,and proteins were added into the enzyme solution to increase the stability of D-tagatose 3-epimerase. The effect of different substances on storage stability of Dtagatose 3-epimerase( DTE) was studied through single factor experiments and orthogonal experiment. The results showed that 0. 3% methylparaben( Met) was effective to control the growth and reproduction of microbes and reduce the losing rate of enzyme activity significantly. Fructose,inositol and Mg SO4 were relatively good stabilizers. The complex stabilizers consist of 2 mmol / L Mg SO4,6% fructose,and 4% inositol. When the enzyme solution containing0. 3% Met and complex stabilizers was stored at 25 ℃,its half-life was 62 days,while its half-life would be 25 days if only 0. 3% Met was added into enzyme solution during storage. The half-life of the control enzyme was only 2 days.Thus complex stabilizers and preservatives were helpful to improve the storage stability of D-tagatose 3-epimerase.