In order to determine the purification performance of titanium dioxide( TiO2) to phosvitinphosphopeptides( PPPs),we investigated the optimum conditions of PPPs purification and inhibiting ability on calcium precipitation. The results showed that the optimum condition for PPPs purification was as follows: p H 4. 0,time 20 min,initial peptides concentration 40 mg / m L,reaction temperature 25 ℃. The purity of the optimized PPPs was high( N / P5. 34) and the effect of inhibiting calcium precipitation was obvious. The study not only provided a new way for the comprehensive utilization of defatted egg yolk protein but also provided a new research direction for the purification of PPPs.