A strain producing L-erythrulose from erythritol was isolated from rotted vegetables. A series of analyses on morphological and biochemical characteristics and sequence analysis of 16 S rRNA revealed that it belonged to Gluconobacter kondonii,which named Gluconobacter kondonii HD385. A preliminary study on fermentation showed that the strain produced 82. 9 g / L of L-erythrulose using 10% erythritol in a medium as carbon source.