In order to obtain yeast suitable for the fermentation of blueberry wine,a total of 122 yeast strains were isolated from different blueberry fruits,leaves,and orchard soil. After screening with microscopy and duchenne tube fermentation,a yeast designated as BBF-17 was found to show excellent tolerance and alcoholic yield compared with the others and K yeast. Based on 18 S r DNA sequences analysis and phylogenetic analysis,the results from molecular biological identification showed that it was very similar to Saccharomyces cerevisiae( EU649672. 1) with similarity of 99%. So the strain was identified as Saccharomyces cerevisiae.