Cellulose is the richest renewable resource in the world,and it has been a difficult and hot spot for research to break down into small molecular sugars and turn them into fuel or fine chemicals.Cellobiohydrolase is one of the key enzymes of cellulose biodegradation,which belongs to excision enzyme.It acts on the end of the chain of crystalline cellulose and cuts the separated beta-1,4-glycosidic bond,finally releasing cellobiose.In this article,the source,classification,structure,the action mechanism,enzymatic properties of cellobiohydrolases,molecular evolution,production situation of commercial enzymes and their application characteristics are summarized.In addition,the research perspective on cellobiohydrolasesis proposed.