In order to reach the "THREE-HIGH"(High-titer,High-productivity and High-conversion) technical levels of enzymatic hydrolysisat low cellulase loading,the enzymatic hydrolysis at high solid concentration was conducted.The enzymatic hydrolysis conditions were optimized enzyme loading 6 FPU/g substrate,substrate concentration150 g/L) and the suitable additive(Tween 80 30 mg/g substrate.Further,a fed-batch strategy was adopted to make the final solid concentration up to 35%,followed by detecting a reasonable cellulase addition way.Finally,the final glucose yield achieved 132 g/L at 72 h from the fed-batch thick-mash enzymatic hydrolysis,with 60% of the cellulose conversion.Results indicate that the atmospheric alkali-catalyzed glycerol pretreated substrate very applicable and attractive for the thick-mash enzymatic hydrolysis at a low cellulase loading.The feedbatcha preferable way to realize "THREE-HIGH" technological level of thick-mash enzymatic hydrolysis of lignocellulosic biomass.