The Eucalyptus leaves lignin degradation by Trichoderma spp.LaTr01 and Ganoderma lucidum TR6 from three aspects the degradation of Eucalyptus leaves,the adsorption and degradation of pure enzyme and Eucalyptus lignin,and the changes of substrate and product.The results prove that the degradation degree of residual lignin was closely related to the laccase producing ability.In the process of solid-state fermentation with Eucalyptus leaves residue as substrate,the laccase activity of TR6 was higher,LaTr01 showed the characteristics of high expression,high excretion rate and rapid enzyme production.The adsorption of two kinds of purified laccase on purified Eucalyptus lignin was close to the Uniform Surface Two-Step Adsorption Model,which conformed to Sips adsorption model adsorption capacity of TR6 was higher and the effect was more obvious.Spectroscopic analysis of the substrate and detection of the product by GC-MS showed that there was no difference in the number of functional groups in the substrates of the two kinds of laccase,the degradation pathway of both the side chain oxidative demethylation and the aromatic ring skeleton breakage.The differences of the degradation of Eucalyptus leaves lignin by two kinds of fungal laccase mainly showed that the molecular structure of laccase led to the accessibility of the substrate,which further led to the enzymatic hydrolysis rate the type and quantity of products.