The 70-day old IRA rabbit spine,leg bones and ribs were used to prepare fine rabbit bone meal,superfine rabbit bone meal,ultramicro rabbit bone meal and nano rabbit bone meal by a series pretreatments and different processing.The general nutrients and amino acids of different sizes of rabbit bone were compared,the calcium release and chemical structure,physicochemical properties of different size of rabbit bone were also compared.The results show that nano rabbit bone meal were rich in nutrients,calcium content was(6.27 ± 0.09) %,which shows the bone can be used as a high quality calcium source.The analysis of FT-IR map shows no significant difference in chemical structure between rabbit bone and nano particle size of rabbit bone.The calcium release of nano rabbit bone meal is the highest of 29.25%,indicating calcium of nano rabbit bone meal is more easily absorbed.The study can provide a theoretical basis for the deep processing of rabbit bone and other animal bones.