For efficient preparation of rare ginsenoside Gyp17 and Gyp75 from ginsenoside Rb1, a ginsenosidase type III was cloned and expressed in Escherichia coli. The results showed that when 20 g/L ginsenoside Rb1 was enzymatically catalyzed at 37 ℃ for 24 h, 3.1 g rare ginsenoside Gyp17 monomer was obtained from 4 g Rb1; with a molar yield of 90.8%. When 5 g/L ginsenoside Rb1 was enzymatically catalyzed at 37 ℃ for 60 h, 2.1 g Gyp75 and 0.45 g Gyp17 monomers were obtained from 4 g Rb1; with a molar yields of 74.2% for Gyp75, and 13.1% for Gyp17. The purities of resultant Gyp17 and Gyp75 were both over 90% according to HPLC analysis. The results provide references for industrial production of Gyp75 and Gyp17.
ZHUANG Ziyu
,
LIU Chunying
,
LI Qiuhong
,
LI Pengfei
,
XU Longquan
,
YU hongshan
. Preparation of rare ginsenoside Gyp17 and Gyp75 by ginsenosidase type III[J]. Food and Fermentation Industries, 2020
, 46(21)
: 42
-47
.
DOI: 10.13995/j.cnki.11-1802/ts.024092
[1] CHRISTENSEN L P.Ginsenosides chemistry,biosynthesis,analysis,and potential health effects[J].Advances in Food and Nutrition Research,2009,55:1-99.
[2] 刘春莹, 宋建国,李鹏飞,等.3种人参中的皂苷的组成[J].大连工业大学学报,2011,30(2):79-82.
[3] SUN H,YANG Z,YE Y.Structure and biological activity of protopanaxatriol-type saponins from the roots of Panax notoginseng[J].International Immunopharmacoogyl,2006,6(1):14-25.
[4] XIA R,ZHAO B,WU Y,et al.Ginsenoside Rb1preconditioning enhances eNOS expression and attenuate smyocardial ischemia/reperfusion injury in diabetic rats[J].Journal of Biomedicine and Biotechnology,2011(2):767930(1-8).
[5] WANG Y,LIU J,ZHANG Z,et al.Anti-neuroinflammation effect of
ginsenoside Rb1 in a rat model of Alzheimer disease[J].Neuroscience Letters,2011,487(1):70-72.
[6] RIVERA E,PETTERSSON F E,INGANAS M,et al.The Rb1 raction of ginseng elicits a balanced Th1 and Th2 immune response[J].Vaccine,2005,23(46-47):5 411-5 419.
[7] YANG C Y,WAN G J,ZHAO Y,et al.Anti-diabetic effects of Panaxnoto ginseng saponins and its major anti-hyperglycemic components[J].Journal of Ethnopharmacology,2010,130(2):231-236.
[8] LEUNG K W,WONG A S.Pharmacology of ginsenosides:A literature review[J].Chinese Medicine,2010,5(1):20-22.
[9] LEUNG K W,CHEUNG L W T,PON Y L,et al.Ginsenoside Rb1 inhibits tube-like structure formation of endothelial cells by regulating pigment epithelium-derived factor through the oestrogen beta recepto[J].British Journal of Pharmacology,2007,152(2):207-215.
[10] CAI B X,JIN S L,LUO D,et al.Ginsenoside Rb1 suppresses ultraviolet radiation-induce dapoptosis by inducing DNA repair[J].Biological and Pharmaceutical Bulletin,2009,32(5):837-841.
[11] TAWAB M A,BAHR U,KARAS M,et al.Degradatin of ginsenosides in humans after administration[J].Drug Metabolism and Disposition,2003,31(8):1 065-1 071.
[12] PARK C S,YOO M H,NOH K H.Biotrasformation of ginsenoside by hydrolyzing the sugar moieties of ginsenoside using microoganism[J].Applied Microbiology and Biotechnology,2010,87:9-19.
[13] CHOI S H,SHIN T J,HWANG S H,et al.Differential effects of ginsenoside metabolites on HERG k channel currents[J].Journal of Ginseng Research,2011,35(2):191-199.
[14] LEE J H,AHN J Y,SHIN T J,et al.Effects of minor ginsenosides,ginsenoside metabolites,and ginsenoside epimers on the growth of Caenorhabditis elegans[J].Journal of Ginseng Research,2011,35(3):375-383.
[15] WANG D M,YU H S,SONG J G,et al.A novel ginsenosidase from an Aspergillus strain hydrolyzing 6-O-multi-glycosides of protopanaxatriol-type ginsenosides,named ginsenosidase type IV[J].Journal of Microbiology and Biotechnology,2011,21(10):1 057-1 063.
[16] 沈宏伟, 肖彦春,车仁国,等.绞股蓝化学成分研究的现状[J].时珍国医国药,2008,19(7):1 561-1 564.
[17] LIU C Y,ZHOU R X,SUN C K,et al. Preparation of minor ginsenoside C-Mc,C-Y,F2 and C-K from American ginseng PPD-ginsenoside using special ginsenosidase type-I from Aspegillus niger g.848[J].Journal of Ginseng Research,2015,39(3):221-229.
[18] YU H,LIU H,ZHANG C,et al. Purification and characterization of gypenoside-α-L-rhamnosidase hydrolyzing gypenoside-5 into gisenoside Rd[J].Process Biochemistry,2004,39:861-867.
[19] JIN X F,YU H S,WANG D M,et al.Kinetics of a cloned special ginsenosidase hydrolyzing 3-O-glucoside of multi-protopanaxadiol-type ginsenosides,named ginsenosidase type III[J].Journal of Microbiology and Biotechnology,2012,22(3):343-351.
[20] AN D S,CUI C H,LEE H G,et al.Identification and characterization of a novel Terrabacter ginsenosidimutans sp.nov.glucosidase that transforms ginsenoside Rb1 into the rare gypenosides XVII and LXXV[J].Applied and Environmental Microbiology,2010,76(17):5 827-5 836.
