To improve the detection efficiency and decrease the cost, a duplex LAMP-HRM (loop mediated isothermal amplification-high resolution melting) method was established for the detection of cow milk components in goat milk. Through the optimization of primer design, the optimized Tm value of cow and goat amplicons were 80 ℃ and 83 ℃, respectively. The two melting peaks were overlap in traditional melting curve assay, but can be easily distinguished by the proposed LAMP-HRM method. This method could simultaneously detect the cow and goat components, and there was no cross-reactivity and false positives for other species. Moreover, the adulteration ratio could be quantified by the height of the melting peak, and it is applied to the detection of goat milk and its dairy products.
ZHANG Wenjuan
,
TANTAI Wei
,
LI Minkang
,
CAI Luyang
,
XU Qinfeng
. Determination of cow milk components in goat milk by LAMP-HRM[J]. Food and Fermentation Industries, 2020
, 46(21)
: 223
-227
.
DOI: 10.13995/j.cnki.11-1802/ts.024338
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