To clarify the key pathways and the metabolic flux distribution of the competing branches of seven Klebsiella strains duringcotton stalk hydrolyzed sugar liquid fermentation to produce succinic acid,and to achieve high-efficiency regulation,the contents of succinic acid,glucose and xylose,the activities of succinic acid dehydrogenase and phosphoenolpyruvate carboxylase,and the concentrations of pyruvate and lactic acid of each strain at 6,12,18,24,30 and 36 h were monitored,and the metabolic characteristics were clarified.The results showed that all Klebsiella strains could effectively ferment cotton stalk to produce succinic acid in 6-36 h,and the fermentation time of succinic acid production was mostly in 6-12 h.WL1309 and WL1312 obtained higher succinic acid production (>42 g/L) at 6 h,and the succinic acid content of the other strains peaked at 12 h of fermentation.By the end of fermentation (36 h),the glucose utilization rate of all strains was higher than 85%,and the utilization rate of xylose was higher than 90%.The succinate dehydrogenase activity of each strain was low.The phosphoenolpyruvate carboxylase mainly affected the synthesis of succinic acid at 6-12 h and 24-36 h.The pyruvate node may accelerate the conversion of succinic acid in the middle and late stages of fermentation (24~36 h),causing the decrease of the metabolic flux distribution of the succinic acid synthesis branch,while the influence of lactic acid on the metabolic flux distribution was weak.During the fermentation process,the high yield of succinic acid can be achieved to a certain extent by regulating the key enzymes and major metabolites in the fermentation process.
LIU Panpan
,
CUI Zhiyong
,
WANG Zikun
,
ZHANG Qin
. Metabolic characteristics of succinic acid production from cotton stalk hydrolysate by Klebsiella strains[J]. Food and Fermentation Industries, 2020
, 46(22)
: 8
-13
.
DOI: 10.13995/j.cnki.11-1802/ts.024572
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