A strain with high production of extracellular polysaccharides,Streptococcus thermophilus MN-002,was selected from traditional fermented dairy products.To further explore the efficacy of S.thermophilus MN-002 in relieving body fatigue,108 male mice were randomly divided into three groups,and were administered by saline,MN-002 fermented milk and MN-002 powder for 45 d.The anti-fatigue effect of S.thermophilus MN-002 was analyzed through the weight-bearing swimming test of mice and the detection of blood lactic acid,serum urea and liver glycogen indexes.The results showed that both fermented milk and bacterial powder significantly improved the exercise tolerance of mice,increased hepatic glycogen,and reduced blood lactate content and serum urea nitrogen.Therefore,it was concluded that MN-002 fermented milk and powder had the effect of relieving physical fatigue,and the effective dose was 2×108CFU/d.This provides a theoretical basis for the application of S.thermophilus MN-002 in anti-fatigue and product development.
HAN Yuting
,
CHEN Jianguo
,
LI Zhouyong
,
LUAN Shaomeng
,
KANG Xiaohong
. In vivo anti-fatigue effect of Streptococcus thermophilus MN-002 fermented milk and bacterial powder in mice[J]. Food and Fermentation Industries, 2020
, 46(22)
: 113
-117
.
DOI: 10.13995/j.cnki.11-1802/ts.024546
[1] FUKUDA K,STRAUS S E,HICKIE I,et al.The chronic fatigue syndrome:A comprehensive approach to its definition and study.International Chronic Fatigue Syndrome Study Group[J].Ann Intern Med,1994,121(12):953-959.
[2] AKAZAWA K H,CUI Y,TANAKA M,et al.Mapping of regional brain activation in response to fatigue-load and recovery in rats with c-Fos immunohistochemistry[J].Neurosci Res,2010,66(4):372-379.
[3] CHAUDHURI A,BEHAN P O.Fatigue in neurological disorders[J].Lancet,2004,363(9 413):978-988.
[4] DUBOC P,MOLLET B.Applications of exopolysaccharides in the dairy industry[J].Int Dairy J,2001,11(9):759-768.
[5] RUASMADIEDO P,HUGENHOLTZ J,ZOON P.An overview of the functionality of exopolysaccharides produced by lactic acid bacteria[J].Int Dairy J,2002,12(2):163-171.
[6] KANG X,LING N,SUN G,et al.Complete genome sequence of Streptococcus thermophilus strain MN-ZLW-002[J].Journal of Bacteriology,2012,194(16):4 428-4 429.
[7] 康小红,张兰威,孙国庆,等.一种嗜热链球菌胞外多糖及其制备和检测方法:中国,CN102702372B[P].2012-10-03.
[8] 王凡,陈建国,李周勇,等.甘南藏族传统发酵乳中高产胞外多糖菌株筛选及发酵特性研究[J].食品科技,2019,44(7):10-15.
[9] LIU J,DU C,WANG Y,et al.Anti-fatigue activities of polysaccharides extracted from Hericium erinaceus[J].Experimental and Therapeutic Medicine,2015,9(2):483-487.
[10] WYBENGA D R,GIORGIO J D,PILEGGI V J.Manual and automated methods for urea nitrogen measurement in whole serum[J].Clin Chem,1971,17(9):891-895.
[11] SOTELO-FÉLIX J I D,MARTINEZ-FONG D,MURIEL P,et al.Evaluation of the effectiveness of Rosmarinus officinalis (Lamiaceae) in the alleviation of carbon tetrachloride?induced acute hepatotoxicity in the rat[J].J Ethnopharmacol,2002,81(2):145-154.
[12] SHANG Y,CHENG J,QI J,et al.Scutellaria flavonoid reduced memory dysfunction and neuronal injury caused by permanent global ischemia in rat[J].Pharmacol Biochem Behav,2005,82(1):67-73.
[13] ZHANG Gang,ZHOU Simin,TIAN Junhuai,et al.Anti-fatigue effects of methazolamide in high-altitude hypoxic mice[J].Tropical Journal of Pharmaceutical Research,2012,11 (2):209-215.
[14] ANAND T,KUMAR G P,PANDAREESH M D,et al.Effect of bacoside extract from Bacopa monniera on physical fatigue induced by forced swimming[J].Phytother Res,2012,26(4):587-593.
[15] XU C,LV J,LO Y M,et al.Effects of oat β-glucan on endurance exercise and its anti-fatigue properties in trained rats[J].Carbohydr Polym,2013,92(2):1 159-1 165.
[16] IKEUCHI M,KOYAMA T,TAKEI S,et al.Effects of benzylglucosinolate on endurance capacity in mice[J].J Health Sci,2009,55(2):178-182.
[17] TANG W,ZHANG Y,GAO J,et al.The anti-fatigue effect of 20 (R)-ginsenoside Rg3 in mice by intranasally administration[J].Biol Pharm Bull,2008,31(1):2 024-2 027.
[18] CHO C W,HAN C J,RHEE Y K,et al.Cheonggukjang polysaccharides enhance immune activities and prevent cyclophosphamide-induced immunosuppression[J].Int J Biol Macromol,2015,72:519-525.
[19] GAO H,ZHANG W,WANG B,et al.Purification,characterization and anti-fatigue activity of polysaccharide fractions from okra (Abelmoschus esculentus (L.) Moench)[J].Food Funct,2018,9(2):1 088-1 101.
[20] WANG J,BAO A,MENG X,et al.An efficient approach to prepare sulfated polysaccharide and evaluation of anti-tumor activities in vitro[J].Carbohydrate Polymers,2018,184:366-375.
[21] 兰美华,吴红彦.中药植物多糖抗氧化作用研究进展[J].实用中医药杂志,2012,28(4):326-327.
[22] 董碧莲,蔡延渠,吕莉,等.中药多糖增强免疫、抗疲劳作用的研究进展[J].2019,41(5):1 119-1 124.
[23] 胡馨予,赵冰,孙晓琪,等.枸杞子多糖抗疲劳活性研究[J].食品科技,2015,40(7):197-200.
[24] 何忠梅,管春红,李盛钰.植物乳杆菌C88联合短梗五加多糖抗疲劳作用[J].2016,41(2):99-103.
[25] 吴铭,周桃英,陈年友,等.黄芪多糖抗疲劳作用研究[J].湖北农业科学,2014,53(1):175-177.
[26] 林华,徐瑞,田明建,等.人参茎叶多糖对大鼠血清自由基清除能力及抗疲劳能力的影响[J].辽宁师范大学学报(自然科学版),2015,38(4):555-560.
