This study explored the immunoregulatory effect of zinc and VC compound formula on cyclophosphamide (CTX)-induced immunosuppression in immature mice.Thirty six three-week old mice were randomly divided into three groups:Control group,CTX group and CTX + complex group.CTX+complex group were treated with zinc citrate and VC,while the other two groups were used as control.On 14 d and 15 d,CTX was applied by intraperitoneal injection as immunosuppressive agent in CTX group and CTX+complex group.After 28 day's administration,immunological parameters were investigated.The results showed that zinc citrate and VC complex could significantly increase the weight of thymus gland and coefficient of mice.Besides,this complex also could increase the concentration of IL-2,TNF-α,IFN-γ in mice's peripheral blood,the content of spleen Th1 cell and the number of Peyer's patch.Meanwhile,the content of SIgA as well as the number of hemolytic plaque in mice's intestine were also be increased,but it had no effect on NK cells activity and lymphocytes proliferation in the immature mice.These results suggested that zinc and VC compound formula could alleviate cyclophosphamide-induced immunosuppression in immature mice by enhancing peripheral immunity.
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