In order to increase the application value of probiotic Lactobacillus casei from fermented dairy products,taking Lactobacillus casei LC-7as the research object,the changes of pH value,titrated acidity,viscosity,viable bacteria number and other characteristics of strain L.caseiLC-7 in the fermentation process,as well as the survival state in the storage period of fermented milk were evaluated,and the volatile flavor substances of fermented milk were determined.The results showed that L.caseiLC-7 entered the stable stage after 24 h at 34 ℃,and possessed fermentation performance.Under this condition,the fermented milk gradually became semi-solid,basically curd,with milk flavor overflow after 14 hours of fermentation.At the end of fermentation,the pH value was 4.67,the titrated acidity was 86.56 °T,and the viscosity of the yogurt was 189 mPa·s.The stability was between 55%-65% under 4 ℃ for a period of time,and the fermented milk was richer in volatile substances than the ordinary fermented yogurt. L.caseiLC-7 grows fast and has good fermentation characteristics,which can be used for yogurt fermentation.
CHI Junxi
,
LEI Wenping
,
LIU Xiaofang
,
LIU Chengguo
. Growth and fermentation characteristics of Lactobacillus casei LC-7 in milk[J]. Food and Fermentation Industries, 2020
, 46(22)
: 208
-213
.
DOI: 10.13995/j.cnki.11-1802/ts.024215
[1] BEAL C,SKOKANOVA J,LATRILLE E,et al.Combined effects of culture conditions and storage time on acidification and viscosity of stirred yogurt[J].Journal of Dairy Science,1999,82(4):673-681.
[2] GISELE I C,RACHAD S,GEORGES C,et al.Characterisation of lactic acid bacteria isolated from fermented milk "laban"[J].International Journal of Food Microbiology,2006,110(1):52-61.
[3] 马佳歌,于微,李佳君,等.干酪乳杆菌细菌素的抗菌机制分析[J].食品科学,2018,39(10):193-198.
[4] DIMITRELLOU D,KANDYLIS P,KOURKOUTAS Y.Assessment of freeze-dried immobilized Lactobacillus casei as probiotic adjunct culture in yogurts[J].Foods,2019,8(9):374.
[5] 胡风林.关于新型酸奶发酵工艺的探讨[J].食品安全导刊,2019,235(11):26.
[6] 黄文利,陈卫,陆英,等.益生菌干酪乳杆菌LC-15生长及发酵特性研究[J].乳业科学与技术,2007,30(6):282-286;295.
[7] 王英,周剑忠,仇小妹,等.益生菌干酪乳杆菌FM10-3生物学与发酵特性分析[J].江苏农业学报,2013,29(3):654-658.
[8] 刘素纯,肖凯.桑葚乳酸菌饮料稳定性研究[J].科技经济导刊,2018,26(33):139-140;142.
[9] 雷文平,周辉,周杏荣,等.SPME-GC-MS结合组学技术分析发酵椰奶特征风味与风味物质相关性[J].食品与机械,2019,35(2):42-47.
[10] 李海平,周登云,付荷蓉,等.滑菇多糖对发酵酸奶品质的影响[J].食品与发酵工业,2019,45(7):188-194.
[11] 蒯月娣,孙月娥.凝固型银杏大枣酸奶在储存过程中的特性研究[J].农业机械,2012(3):106-108.
[12] 郜洪涛,吕嘉枥,闫肃,等.嗜热链球菌对酸奶发酵的影响及应用前景[J].中国酿造,2010(11):5-8.
[13] 孙文伶,赵丽娜,董阳,等.高密度菌种发酵酸奶品质变化研究[J].中国酿造,2018,37(8):133-137.
[14] FALLGREEN-GEBAUER E,GEBAUER W,BASTIAN A,et al.The covalent linkage of secretory component to IgA.structure of sIgA[J].Biol Chem,1993,374(11):1 023-1 028.
[15] 陈冲,宋丽菊,齐盼盼,等.干酪乳杆菌在乳制品中的应用研究进展[J].食品与发酵科技,2015,51(4):88-91.
[16] 泰米迈,罗宾逊.酸乳科学与技术[M].北京:中国农业出版社,2003.
[17] 李广富,陈伟,范路平,等.灵芝功能成分酸奶营养品质与风味物质分析[J].食品科学,2015,36(10):168-173.