A method for the determination of five phytosterols in brown sugar,dark brown sugar and brown granulated sugar by high performance liquid chromatography with diode array detector was established.The sample was extracted by anhydrous ethanol,then saponified in water bath;after that the sample was extracted with dichloromethane,dried with nitrogen and then dissolved with methanol.A Waters ACQUITY UPLC BEH C8 liquid chromatographic was used for the detection with the following conditions:Column (2.1 mm×100 mm,1.7μm);acetonitrile and aqueous solution were used as mobile phase with the flow rate 0.4 mL/min and the column temperature at 35 ℃;the injection volume was 5 μL and the detection range of diode array detector was 190-810 nm;the detection wavelength was 205 and 282 nm.The results showed that the linear correlation coefficients of ergosterol,cholesterol,rapeseed oil sterol,stigmasterol and β-sitosterol in the concentration range of 0.1-100 μg/mL were 0.999 7 and 0.999 9,respectively.The detection limits of the method were 0.023,0.14,0.18,0.11 and 0.17 mg/kg.The recovery rate of the three concentration addition levels was 74.4%-102.5%,and the relative standard deviation is 0.1%-3.6%.The results of sample determination showed that the contents of five sterols and total sterols in the three sugars were significantly different.The overall distribution was brown sugar >dark brown sugar >brown granulated sugar.The method developed here has the advantages of simple pretreatment,high sensitivity,good reproducibility and recovery,good precision and accuracy,and can be used for the analysis and detection of five kinds of phytosterols in sugar.
CHEN Qizhao
,
LIN Yanchun
,
LIU Zhipeng
,
CHEN Jiamin
,
LI Changcheng
,
WANG Guihua
,
LI Jiawei
,
YU Goubin
. Determination of phytosterols in three kinds of sugar[J]. Food and Fermentation Industries, 2020
, 46(22)
: 238
-244
.
DOI: 10.13995/j.cnki.11-1802/ts.024376
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