Microbial community structure and dynamic succession in traditional fermentation ofMucor-type Yongchuan Douchi

  • LI Wei ,
  • LUO Shenbin ,
  • QIU Zerui ,
  • LAI Denglei ,
  • WANG Hongwei ,
  • SUO Huayi
Expand
  • 1(College of Food Science,Southwest University,Chongqing 400715,China);
    2(Chongqing Yongchuan Douchi Food Co.,Ltd.,Chongqing 402160,China)

Received date: 2020-05-27

  Revised date: 2020-06-06

  Online published: 2020-12-30

Abstract

Using high-throughput sequencing method,the community structure and dynamic succession of the microorganisms inMucor-type Yongchuan Douchi during koji making stage and post fermentation stage were analyzed.The results showed that the bacterial diversity in whole process of fermentation was higher than that of fungi,and the diversity of microorganism community during koji making stage was more abundant than that of the post fermentation stage,with more significant community succession.In total,there were 17 fungal and 25 bacterial genera with relative abundance more than 5%.During koji making stage,the dominant fungi consisted of MucorandPenicillium, and the dominant bacteria wereLeuconostoc and Weissella.During the post fermentation stage, Aspergillus, Penicillium, Bacillus, Delftia and Rhizobiumhad relatively higher abundance,while a few fungi,which are mainlyMucor,played a key role with relatively lower abundance.Bacillus,Staphylococcus andCorynebacteriumwere crucial to the whole fermentation process.The result lays a foundation for the regulation and quality control of theMucor-type Douchi and the development of composite fermentation agent.

Cite this article

LI Wei , LUO Shenbin , QIU Zerui , LAI Denglei , WANG Hongwei , SUO Huayi . Microbial community structure and dynamic succession in traditional fermentation ofMucor-type Yongchuan Douchi[J]. Food and Fermentation Industries, 2020 , 46(23) : 60 -67 . DOI: 10.13995/j.cnki.11-1802/ts.024566

References

[1] YOSHIKAWA Y,CHEN P Y,ZHANG B,et al.Evaluation of seed chemical quality traits and sensory properties of natto soybean[J].Food Chemistry,2014,153:186-192.
[2] 索化夷,赵欣,骞宇,等.永川豆豉发酵过程中质构色泽形成规律[J].食品与发酵工业,2016,42(7):80-85.
SUO H Y,ZHAO X,QIAN Y,et al.Texture and color formation of Yongchuan Douchi during fermentation process[J].Food and Fermentation Industries,2016,42(7):80-85.
[3] 王鹏娇,吴运莉,张敏,等.21种不同产地中药淡豆豉中5种活性成分的对比[J].中国实验方剂学杂志,2014,19:64-67.
WANG P J,WU Y L,ZHANG M,et al.Analysis of five active ingredients in 21 Semen Sojae Praeparatum of traditional Chinese medicine from different regions[J].Chinese Journal of Experimental Traditional Medical Formulae,2014,19:64-67.
[4] 阚建全,陈宗道,石轶松,等.豆豉非透析类黑精抗氧化和抑制亚硝胺合成的研究[J].营养学报,1999(3):349-352.
KAN J Q,CHEN Z D,SHI Y S,et al.Study on antioxidation and inhibition of nitrosamine synthesis by Douchi nondialyzable melanoidin[J].Acta Nutrimenta Sinica,1999(3):349-352.
[5] LI F J,YIN L J,LU X,et al.Changes In angiotensin I-converting enzyme inhibitory activities during the ripening of Douchi(a Chinese traditional soybean product) fermented by various starter cultures[J].International Journal of Food Properties,2010,13(3):512-524.
[6] HE G Q,HUANG J,LIANG R,et al.Comparing the differences of characteristic flavour between natural maturati on and starter culture forMucor-type Douchi[J].International Journal of Food Science and Technology,2016,51(5):1 252-1 259.
[7] 任聪,杜海,徐岩.中国传统发酵食品微生物组研究进展[J].微生物学报,2017,57(6):885-898.
REN C,DU H,XU Y.Advances in microbiome study of traditional Chinese fermented foods[J].Acta Microbiologica Sinica,2017,57(6):885-898.
[8] PETROSINO J F,HIGHLANDER S,LUNA R A,et al.Metagenomic pyrosequencing and microbial identification[J].Clinical Chemistry,2009,55(5):856-866.
[9] 谭强来,曾臻,吴兰兰,等.基于高通量测序的江西特色发酵豆豉中微生物群落多样性及其特征分析[J].食品工业科技,2019,40(3):85-89;96.
TAN Q L,ZENG Z,WU L L,et al.Diversity and characteristics of microbial community in fermented Douchi from Jiangxi province using high-throughput sequencing technology[J].Science and Technology of Food Industry,2019,40(3):85-89;96.
[10] 石聪,李世瑞,李跑,等.基于高通量测序浏阳豆豉不同发酵阶段微生物多样性分析[J].食品与发酵工业,2018,44(2):27-32;39.
SHI C,LI S R,LI P,et al.Analysis of microbial diversity in different fermented stages of Liuyang Douchi based on high throughput sequencing[J].Food and Fermentation Industries,2018,44(2):27-32;39.
[11] 董蕴,许小玲,代凯文,等.基于Miseq高通量测序技术对细菌型豆豉细菌类群的评价[J].食品工业科技,2018,39(13):150-154;247.
DONG Y,XU X L,DAI K W,et al.Characterization of bacterial microflora of bacteria-fermented Douchi by Miseq high throughput sequencing[J].Science and Technology of Food Industry,2018,39(13):150-154;247.
[12] 李晓然,李洁,刘晓峰,等.利用高通量测序分析云南豆豉中细菌群落多样性[J].食品与生物技术学报,2014,33(2):137-142.
LI X R,LI J,LIU X F,et al.Analysis of bacterial community diversity in fermented soybean using pyrosequencing[J].Journal of Food Science and Biotechnology,2014,33(2):137-142.
[13] 曾涛,罗沈斌,索化夷.永川毛霉型豆豉在发酵过程中菌群动态变化规律[J].中国调味品,2019,44(3):42-45;64.
ZENG T,LUO S B,SUO H Y.Dynamic change rule of microflora in Yongchuan Mucor-typefermented soya beans during fermentation[J].China Condiment,2019,44(3):42-45;64.
[14] 赵文鹏,李浩,杨慧林,等.曲霉型豆豉发酵阶段细菌群落的演替及其与环境因子的关系[J/OL].食品科学,2020.DOI:10.7506/spkx1002-6630-20191120-233.
ZHAO W P,LI H,YANG H L,et al.Bacterial community succession during the fermentation stage of Aspergillus-type Douchi and its relation with environmental factors[J/OL].Food Science,2020.DOI:10.7506/spkx1002-6630-20191120-233.
[15] RIVAS M N,BURTON O T,WISE P,et al.A microbiota signature associated with experimental food allergy promotes allergic sensitization and anaphylaxis[J].Journal of Allergy and Clinical Immunology,2013,131(1):201-212.
[16] JIANG X T,PENG X,DENG G H,et al.Illumina sequencing of 16S rRNA tag revealed spatial variations of bacterial communities in a mangrove wetland[J].Microbial Ecology,2013,66(1):96-104.
[17] 孙炜宁.基于高通量测序的泸酒酒醅和酸菜中细菌区系的研究[D].天津:天津大学,2017.
SUN W N.Bacterial diversity research of Luzhou-flavor liquor and Suancai based on Illumina Miseq sequencing[D].Tianjin:Tianjin University,2017.
[18] PRICE M N,DEHAL P S,ARKIN A P.Fast tree 2-approximately maximum-likelihood trees for large alignments[J].Plos One,2010,5(3).
[19] 汪孟娟,熊顺强,陈廷涛,等.PCR-DGGE监测豆豉制曲过程中菌群的动态变化[J].南昌大学学报(理科版),2010,34(6):571-574.
WANG M J,XIONG S Q,CHEN T T,et al.Dynamic changes of flora in koji-making Douchi by using PCR-denaturing gradient gel electrophoresis[J].Journal of Nanchang University(Natural Science),2010,34(6):571-574.
[20] WANG X S,DU H,XU Y.Source tracking of prokaryotic communities in fermented grain of Chinese strong-flavor liquor[J].International Journal of Food Microbiology,2017,244:27-35.
[21] CROWLEY S,MAHONY J,VAN S D.Current perspectives on antifungal lactic acid bacteria as natural bio-preservatives[J].Trends in Food Science and Technology,2013,33(2):93-109.
[22] RHEE S J,LEE J,LEE C.Importance of lactic acid bacteria in Asian fermented foods[J].Microbial Cell Factories,2011,10.DOI:10.1186/1475-2859-10-S1-S5.
[23] ZHANG W B,LUO Q Q,ZHU Y,et al.Microbial diversity in two traditional bacterial Douchi from Gansu province in northwest China using Illumina sequencing[J].PloS One,2018,13(3).DOI:10.1371/journal.pone.0194876.
[24] HE B,LI H R,HU Z H,et al.Difference in microbial community and taste compounds betweenMucor-type and Aspergillus-type Douchi during koji-making[J].Food Research International,2019,121:136-143.
[25] 李恒,陈功,伍亚龙,等.高通量测序方法研究传统四川泡菜母水中微生物群落的动态变化[J].食品科学,2018,39(24):131-138.
LI H,CHEN G,WU Y L,et al.Analysis of microbial community dynamics of traditional Sichuan Paocai brine by high-throughput sequencing[J].Food Science,2018,39(24):131-138.
[26] KIM T W,LEE J H,KIM S E,et al.Analysis of microbial communities in doenjang,a Korean fermented soybean paste,using nested PCR denaturing gradient gel electrophoresis[J].International Journal of Food Microbiology,2009,131(2-3):265-271.
[27] KIM T W,LEE J H,PARK M H,et al.Analysis of bacterial and fungal communities in Japanese-and Chinese-fermented soybean pastes using nested PCR-DGGE[J].Current Microbiology,2010,60(5):315-320.
[28] 索化夷,骞宇,阚建全.传统发酵永川毛霉型豆豉蛋白质提取方法比较与降解规律研究[J].食品科学,2013,34(23):180-183.
SUO H Y,QIAN Y,KAN J Q.Comparison of methods for protein extraction from traditional fermented Yongchuan Douchi and degradation pattern[J].Food Science,2013,34(23):180-183.
Outlines

/