Taxonomic identification and virulence gene verification of 7Escherichia coli strains were carried out,and the feasibility of using them as the standard strains of GB 4789.6—2016 “Microbiological examination of food—Examination of diarrheogenicE.coli”was evaluated.Seven strains ofE.coli were identified by polyphase-taxonomy identification method;according to the methods of GB 4789.6—2016 standard,virulence gene verification of 7 strains of E.coli were carried out.Results showed that the strain CICC 10003 carried geneuidA;CICC 10667 carried genesuidA, sthand lt;CICC 10670 and CICC 24187 carried genesuidA,escV,stx1 andstx2;CICC 24186 carried genesuidA,pic,astA andaggR;CICC 24188 carried genesuidA andinvE;CICC 24189 carried genesuidA,bfpB andescV.The strains CICC 10667,CICC 24186,CICC 24188 and CICC 24189 were identified as enterotoxigenicE.coli (ETEC),enteroaggregativeE.coli (EAEC),enterotoxigenicE.coli (EIEC) and enteropathogenicE.coli (EPEC),respectively;CICC 10670 and CICC 24187 belonged to enterohemorrhagicE.coli(EHEC);CICC 10003 belonged to non-diarrheogenicE.coli.The performance of the 7 strains met the requirements of the corresponding types ofE.coliin the GB 4789.6—2016 standard,and they can be used as standard strains for the examination of diarrheogenicE.coli.
ZHANG Caiwen
,
LI Jinxia
,
CHEN Yiwen
,
DU Haibo
,
LIU Jiaqi
,
XIN Di
,
SHI Liang
,
CUI Shenhui
,
YAO Su
. Study of standard strain for testing diarrheagenicEscherichia coli[J]. Food and Fermentation Industries, 2020
, 46(23)
: 68
-73
.
DOI: 10.13995/j.cnki.11-1802/ts.025322
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