Advances on polyphasic classification and identification ofAspergillus nigerisolates

  • HAN Xiaomin ,
  • LI Fengqin
Expand
  • 1(NHC Key Laboratory of Food Safety Risk Assessment,China National Center for Food Safety Risk Assessment,Beijing 100021,China)

Received date: 2020-04-30

  Revised date: 2020-05-11

  Online published: 2020-12-30

Abstract

Aspergillus nigeris commonly used in food fermentation industry.It was also found in food spoilage.Precise classification and identification ofA.niger isolates are the research hotspot for taxonomists.At present,besides traditional morphological identification,polyphasic classification methods based on features of physiology,extrolites,and molecular biology are exploited in the classification and identification ofA.niger strains.The advantages and disadvantages of each method and its practical application in the identification ofA.niger strains are elaborated in detail.The future development direction about the classification and identification ofA.nigerstrains is put forward.

Cite this article

HAN Xiaomin , LI Fengqin . Advances on polyphasic classification and identification ofAspergillus nigerisolates[J]. Food and Fermentation Industries, 2020 , 46(23) : 279 -285 . DOI: 10.13995/j.cnki.11-1802/ts.024324

References

[1] 齐祖同.中国真菌志:第五卷曲霉属及其相关有性型[M].北京:科学出版社,1997:91-103.
QI Z T.Annals of Chinese fungi:Volume 5Aspergillus and its associated sexual types[M].Beijing:Science Press,1997:91-103
[2] VARGA J,KEVEI F,HAMARI Z,et al.Genotypic and phenotypic variability among black aspergilla.In:Integration of modern taxonomic methods forPenicillumandAspergillus classification.(Samson RA,Pitt JI,eds)[M].Amsterdam:Harwood Academic Publishers,2000,397-411.
[3] FAO/WHO.Toxicological evaluation of certain food additives[C].The 31 st meeting of the Joint FAO/WHO Expert Committee on Food Additives,World Health Organization,Geneva,1987.
[4] FAO/WHO.Toxicological evaluation of certain food additives[C].The 35th meeting of the Joint FAO/WHO Expert Committee on Food Additives,World Health Organization,Geneva,1990.
[5] FAO/WHO.Compendium of food additive specifications,vol 1 and 2.Food and Nutrition Paper no.51/1 and 51/2.Food and Agricultural Organization of the United Nations,World Health Organization,Rome,1992.
[6] 郭鲁宏,杨顺楷.黑曲霉产生的酶类及其应用[J].天然产物研究与开发,1997,10(4):87-93.
GUO L H,YANG S K.Enzymes produced byAspergillus nigerand their applications[J].Natural Product Research and Development,1997,10(4):87-93.
[7] FUNGARO M H P,FERRANTI L S,MASSI F P,et al.Aspergillus labruscus sp.nov.a new species ofAspergillus sectionNigridiscovered in Brazil[J].Scientific Reports,2017,7(1):6 203.
[8] SUSCA A,PROCTOR R H,BUTCHKO R A E,et al.Variation in the fumonisin biosynthetic gene cluster in fumonisin-producing and nonproducing blackaspergilli[J].Fungal Genetics and Biology,2014,73:39-52.
[9] NOONIM P,MAHAKARNACHANAKUL W,NIELSEN K F,et al.Fumonisin B2 production byAspergillus niger in Thai coffee beans[J].Food Additives and Contaminants,2009,26(1):94-100.
[10] FERRACIN L M,FRISVAD J C,TANIWAKI M H,et al.Genetic relationships among strains of theAspergillus nigeraggregate[J].Brazilian Archives of Biology and Technology,2009,52:241-248.
[11] SØÜRENSEN A,LÜBECK P S,LÜBECK M,et al.Aspergillus saccharolyticus sp.nov.,a new blackAspergillus species isolated in Denmark[J].International Journal of Systematic and Evolutionary Microbiology,2011,61(Pt12):3 077-3 083.
[12] ABARCA M L,ACCENSI F,CANO J,et al.Taxonomy and significance of blackaspergilla[J].Antonie Van Leeuwenhoek,2004,86(1):33-49.
[13] SAMSON R A,NOONIM P,MEIJER M,et al.Diagnostic tools to identify blackaspergilla[J].Studies in Mycology,2007,59:129-145.
[14] VARGA J,FRISVAD J C,KOCSUBÉS,et al.New and revisited species inAspergillussectionNigri[J].Studies in Mycology,2011,69:1-17.
[15] SAMSON R A,VISAGIE C M,HOUBRAKEN J,et al.Phylogeny,identification and nomenclature of the genus Aspergillus[J].Studies in Mycology,2014,78:141-173.
[16] FRISVAD J C,LARSEN T O,VRIES R de,et al.Secondary metabolite profiling,growth profiles and other tools for species recognition and importantAspergillus mycotoxins[J].Studies in Mycology,2007,59:31-37.
[17] MURAKAMI H.A brief history of classification of the blackaspergilliincluding the Kuro-koji molds.Taxonomic studies on Japanese industrial strains of theAspergillus (Part 24)[J].Journal of the Society of Brewing Japan,1976,71:952-956.
[18] MURAKAMI H,YOSHIDA K.Grading of morphological characters of the black aspergilli.Taxonomic studies on Japanese Industrial strains of theAspergillus (Part 27)[J].Journal of the Society of Brewing Japan,1979,74:328-331.
[19] MURAKAMI H,YOSHIDA K.Grading of physiological characters of the blackaspergilli.Taxonomic studies on Japanese Industrial strains of theAspergillus (Part 28)[J].Journal of the Society of Brewing,Japan,1979,74:459-461.
[20] MURAKAMI H,YOSHIDA K,YOSHIDA K,et al.Tables of mycological characters of the representative strains of the blackaspergilli.Taxonomic studies on Japanese industrial strains of theAspergillus (Part 30)[J].Journal of the Society of Brewing Japan,1979,74:466-470.
[21] AL-MUSALLAM A.Revision of the blackAspergillus species[D].Utrecht:Utrecht University,Baarn,1980.
[22] PERRONE G,VARGA J,SUSCUA A,et al.Aspergillus uvarum sp.nov.an uniseriate blackAspergillus species isolated from grapes in Europe[J].International Journal of Systematic and Evolutionary Microbiology,2008,58(4):1 032-1 039.
[23] NOONIM P,MAHAKARNCHANAKUL W,VARGA J,et al.Two novel species ofAspergillus sectionNigri from Thai coffee beans[J].International Journal of Systematic and Evolutionary Microbiology,2008,58(7):1 727-1 734.
[24] FUNGARO M H P,FERRANTI L S,MASSI F P,et al.Aspergillus labruscus sp.nov.a new species of Aspergillus sectionNigridiscovered in Brazil[J].Scientific Reports,2017,7(1):6 203.
[25] ROHLFS M,OBMANN B,PETERSEN R.Competition with filamentous fungi and its implication for a gregarious lifestyle in insects living on ephemeral resources[J].Ecological Entomology,2005,30(5):556-563.
[26] FRISVAD J C,BRIDGE P D,ARORA D K.Chemical fungal taxonomy[M].New York:Marcel Dekker,1998.
[27] LARSEN T O,SMEDSGAARD J,NIELSEN K F,et al.Phenotypic taxonomy and metabolite profiling in microbial drug discovery[J].Natural Product Reports,2005,22(6):672-693.
[28] GALAGAN J E,CALVO S E,CUOMO C,et al.Sequencing ofAspergillus nidulans and comparative analysis with A.fumigatus and A.oryzae[J].Nature,2005,438:1 105-1 115.
[29] NIERMAN W H,PAIN A,ANDERSON M J,et al.Genomic sequence of the pathogenic and allergenic filamentous fungus Aspergillus fumigatus[J].Nature,2005,438:1 151-1 156.
[30] PEL H J,DE WINDE J H,ARCHER D B,et al.Genome sequencing and analysis of the versatile cell factoryAspergillus nigerCBS 513.88[J].Nature Biotechnology,2007,25:221-231.
[31] FRISVAD J C,NIELSEN K F,SAMSON R A.Recommendations concerning the chronic problem of misidentification of mycotoxigenic fungi associated with foods and feeds[J].Advances in Experimental Medicine and Biology,2006,571:33-46.
[32] KUSTERS-VAN SOMEREN M A,KESTER H CM,SAMSON R A,et al.Variation in pectinolytic enzymes in blackaspergilli:A biochemical and genetic approach.In:Modern concepts in Penicillium and Aspergillus classification(Samson RA,Pitt JI,eds).[M].New York:Plenum Press,1990,321-334.
[33] KUSTERS-VAN SOMEREN M A,SAMSON R A,VISSER J.The use of RFLP analysis in classification of the blackaspergilli:Reinterpretation of Aspergillus niger aggregate[J].Current Genetics,1991,19(1):21-26.
[34] VARGA J,KEVEI F,FEKETE C,et al.Restriction fragment length polymorphisms in the mitochondrial DNAs of theAspergillus nigeraggregate[J].Mycological Research,1993,97(8):1 207-1 212.
[35] HAMARI Z,KEVEI F,KOVáCA E,et al.Molecular and phenotypic characterization ofAspergillus japonicus andAspergillus aculeatus strains with special regard to their mitochondrial DNA polymorphisms[J].Antonie Van Leeuwenhoek,1997,72:337-347.
[36] MEDINA A,MATEO R,LOPEZ-OCANA L,et al.Study of Spanish grape mycobiota and ochratoxin A production by Isolates ofAspergillus tubingensis and other members ofAspergillus sectionNigri[J].Applied and Environmentral Microbiology,2005,71(8):4 696-4 702.
[37] BAU M,CASTELLA G,BRAGULAT M R,et al.RFLP characterization ofAspergillus nigeraggregate species from grapes from Europe and Israel[J].International Journal of Food Microbiology,2006,111:S18-S21.
[38] MARTINEZ-CULEBRAS P V,RAMON D.An ITS-RFLP method to identify black Aspergillus isolates responsible for OTA contamination in grapes and wine[J].International Journal of Food Microbiology,2007,113(2):147-153.
[39] ZANZOTTO A,BURRUGNO S,MARCIANO P.Digestion of DNA regions to discriminate ochratoxigenic and non-ochratoxigenic strains in the Aspergillus nigeraggregate[J].International Journal of Food Microbiology,2006,110(2):155-159.
[40] PERRONE G,MULE G,SUSCA A,et al.Ochratoxin A production and amplified fragment length polymorphism analysis ofAspergillus carbonarius, Aspergillus tubingensis,andAspergillus nigerstrains isolated from grapes in Italy[J].Applied and Environmentral Microbiology,2006,72(1):680-685.
[41] PERRONE G,SUSCA A,EPIFANI F,et al.AFLP characterization of Southern Europe population ofAspergillussection Nigrifrom grapes[J].International Journal of Food Microbiology,2006,111:S22-S27.
[42] PERRONE G,STEA G,EPIFANI F,et al.Aspergillus nigercontains the cryptic phylogenetic speciesA.awamori[J].Fungal Biology,2011,115(11):1 138-1 150.
[43] SUSCA A,STEA G,PERONE G.Rapid polymerase chain reaction (PCR)-single-stranded conformational polymorphism (SSCP) screening method for the identification of Aspergillus sectionNigrispecies by the detection of calmodulin nucleotide variations[J].Food Additives and Contaminants,2007,24(10):1 148-1 153.
[44] VARAGA J,KOCSUBÉ S,TÓTH B,et al.Aspergillus brasiliensis sp.nov.,a biseriate blackAspergillus species with world-wide distribution[J].International Journal of Systematic and Evolutionary Microbiology,2007,57(Pt 8):1 925-1 932.
[45] YOKOYAMA K,WANG L,MIYAJI M,et al.Identification,classification and phylogeny of the Aspergillus section Nigri inferred from mitochondrial cytochrome b gene[J].FEMS Microbiology Letters,2006,200:241-246.
[46] GEISER D M,KLICH M A,FRISVAD J C,et al.The current status of species recognition and identification inAspergillus[J].Studies in Mycology,2007,59:1-10.
[47] ATOUI A,MATHIEU F,LEBRIHI A.Targeting a polyketide synthase gene forAspergillus carbonarius quantification and ochratoxin A assessment in grapes using real-time PCR[J].International Journal of Food Microbiology,2007,115(3):313-318.
[48] SUSCA A,STEA G,MULE G,et al.Polymerase chain reaction (PCR) identification ofAspergillus niger andAspergillus carbonarius based on the calmodulin gene[J].Food Additives and Contaminants,2007,24(10):1 154-1 160.
[49] PERRONE G,SUSCA A,STEA G,et al.PCR assay for identification ofAspergillus carbonariusandAspergillus japonicus[J].European Journal of Plant Pathology,2004,110(5-6):641-649.
[50] GONZALEZ-SALGADO A,PATNO B,VAZQUEZ C,et al.Discrimination ofAspergillus nigerand otherAspergillus species belonging to sectionNigri by PCR assays[J].FEMS Microbiology Letters,2005,245(2):353-361.
[51] HAUGLAND RA,VESPER S.Method of identifying and quantifying specific fungi and bacteria.US Patent No.6387652[P].2001-07-13.
[52] LEBRIHI A,MATHIEU F,BORGIDA L P,et al.Method for the detection of ochratoxin A-or citrinin-producing fungi.European patent No.EP1329521[P].2003-07-23.
[53] DOBSON A,O″CALLAGHAN J.Detection of ochratoxin A producing fungi.Patent No.WO 2004/072224 A2[P].2004-02-12.
[54] MURAKAMI H,YOSHIDA K,YOSHIDA K,et al.Tables of mycological characters of the representative strains of the blackaspergilli.Taxonomic studies on Japanese industrial strains of theAspergillus(Part 30)[J].Journal of the Society of Brewing Japan,1979,74(7):466-470.
[55] POLLASTRO S,DE MICCOLIS R M,FARETTA F.A new semi-selective medium for the ochratoxigenic fungusAspergillus carbonarius[J].Journal of Plant Pathology,2006,88(1):107-112.
Outlines

/