Food and Fermentation Industries >

2022 , Vol. 48 >Issue 1: 111 - 116

DOI: https://doi.org/10.13995/j.cnki.11-1802/ts.027742

Antimicrobial mechanism of metal antimicrobial peptide SIF4 against Escherichia coli

  • XIAO Huaiqiu ,
  • LI Yuzhen ,
  • LIN Qinlu ,
  • ZHAO Mouming ,
  • ZHOU Quan ,
  • ZHAO Yichun
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  • 1(School of Pharmaceutical and Bioengineering,Hunan Chemical Vocational Technology College,Zhuzhou 412000,China)
    2(College of Food Science and Engineering,Central South University of Forestry and Technology,Changsha 410004,China)
    3(College of Food Science and Engineering,South China University of Technology,Guangzhou 510000,China)

Received date: 2021-04-19

  Revised date: 2021-05-07

  Online published: 2022-01-27

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Abstract

The antimicrobial mechanism of SIF4 against Escherichia coli from cell wall permeability (CWP), intracellular K+ and biomacromolecule leakage, cell surface hydrophobicity (CSH), cell membrane permeability (CMP) and cell surface zeta potential (CSZP) were investigated. Results showed that minimum inhibition concentration of SIF4 was 0.4 mg/L. The stationary phase and decline phase of E.coli came early after treated by SIF4. CWP had a positively correlation with the SIF4 concentration and incubation time, after 1.0 h incubation, there was a significant difference between groups (P<0.05). The leakage of intracellular K+ was enhanced with the increase of SIF4 concentration and incubation time; the leakage of intracellular biomacromolecules also increased with the increase of SIF4 concentration and incubation time, and there was a significant difference between the experimental group and the control group after 2 h incubation (P<0.05). CSH increased gradually with the increase of SIF4 concentration, and the cell membrane damaging caused by SIF4 might be carried out in “carpet model”. The CSZP had a linear decreasing tendency against SIF4 concentration. The research confirmed that SIF4 could destroy cell wall/membrane structure, cause leakage of intracellular substances, enhance CSH, reduce zeta potential, cause cell aggregation and metabolic disorder, and eventually induce cell apoptosis. All results deem that SIF4 could be adopted potentially as a new food antibacterial agent for the biological inhibition of E. coli.

Key words： metal antimicrobial peptide; Escherichia coli; cell wall permeability; cell membrane damage

Cite this article

XIAO Huaiqiu , LI Yuzhen , LIN Qinlu , ZHAO Mouming , ZHOU Quan , ZHAO Yichun . Antimicrobial mechanism of metal antimicrobial peptide SIF4 against Escherichia coli[J]. Food and Fermentation Industries, 2022 , 48(1) : 111 -116 . DOI: 10.13995/j.cnki.11-1802/ts.027742

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