The aim of this study was to explore the effect of royal jelly on immunocompromised mice. Forty-eight male Kunming mice were randomly divided into blank group, cyclophosphamide model group, casein group as well as royal jelly low-dose, medium-dose and high-dose groups, with eight mice in each group. Except for the blank group, the other five groups were intraperitoneally injected with 80 mg/(kg bw) cyclophosphamide every day to establish immunocompromised mouse models. After 30 d of continuous gavage, the body weight, spleen and thymus weight, morphology of spleen were measured. Meanwhile the morphology of spleen and liver, white blood cell count, immunoglobulin content, cytokine level, splenic lymphocyte proliferation ability and testosterone content were observed and by Hematoxylin-Eosin staining. After modeling, the spleen and thymus index were decreased, the morphological structure under the microscope of spleen and liver of mice was disordered. The WBC count, immunoglobulin, IL-6 levels and the proliferation ability of T/B lymphocyte were significantly decreased. However, after the royal jelly intervention, above-mentioned indexes were improved to different extent, and the effect was most significant in the high-dose group. Royal jelly can enhance the immune function of mice with immunodeficiency caused by cyclophosphamide.
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