To reduce the cellulase cost in the production of cellulosic ethanol, Trichoderma reesei TG-C521 with high-yield cellulase was used with steam-exploded corn stover as the carbon source. The influence of dissolved oxygen and reducing sugar on the production and application of cellulase were studied. The results showed that the steam-exploded corn stover had the most significant effect on the cellulase production, followed by corn steep liquor. The optimal medium was as follows: 38.6 g/L of steam-exploded corn stover, 4.8 g/L of (NH4)2SO4, 29.7 g/L of corn steep liquor, 5.3 g/L of bran, 1.0 g/L of KH2PO4, 0.5 g/L of MgSO4. Under the conditions of the dissolved oxygen 20%-30%, reducing sugar 2.0-3.0 g/L and fermentation time 144 h, the cellulase activity reached 39.0 U/mL. The cellulase was further used for in situ saccharification, the cellulose conversion can reach more than 85% with low cellulase loading.
WANG Bei
,
CHEN Jibao
,
YAN Zhenli
,
ZHAO Zigao
,
LI Chao
. Steam-exploded corn stalk as raw substrate for cellulase production[J]. Food and Fermentation Industries, 2022
, 48(1)
: 182
-187
.
DOI: 10.13995/j.cnki.11-1802/ts.029045
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