Determination of glyphosate and glyphosate in tea was carried out by ultra-high performance liquid chromatography-tandem quadrupole time of flight mass spectrometry. Effects of derivation conditions, purification methods and mass spectrometry parameters on the determination of glyphosate and glyphosate in tea were investigated. The results showed that the samples were extracted with pure water enhanced by ultrasound, purified on solid phase extraction column packing mixed cation exchange reversed-phase adsorbent. The purified solution was added into borate buffer solution with pH 10.0 and the derivatization with 9-fluorene methoxycarbonyl chloride acetonitrile solution was carried out for 2 h, then filtered through 0.22 μm microporous membrane. The separation was carried out on an ultra-high performance liquid chromatography BEH C18 column with 2 mmol/L ammonium acetate aqueous solution (containing 0.1% formic acid, V/V) and acetonitrile as mobile phase gradient elution. The targets were detected by quadrupole tandem time-of flight mass spectrometer with electrospray positive ion pseudo-multiple reaction monitoring mode and quantified by external standard method. Glyphosate and glufosinate showed good linearity in the range of 1.25-400 μg/L and 0.25-160 μg/L, respectively (R>0.99). The limits of detection of this method for glyphosate and glufosinate were 4.0 μg/kg, 1.34 μg/kg, respectively. At low, medium and high (2, 10 and 40 times of limits of quantification) supplemental levels, the recoveries of glyphosate ranged from 88% to 103% with relative standard deviations lower than 5%, and glufosinate ranged from 93% to 99% with relative standard deviations lower than 5%. This method is suitable for the determination of glyphosate and glyphosate residues in tea samples with fast analysis speed, good purification effect, low interference from impurities, high recovery rate and good repeatability.
WANG Jun
,
ZHENG Miaokai
,
LI Xiaoting
,
HU Wenmei
,
WANG Zhonghe
. Determination of glyphosate and glufosinate in tea by ultra-high performance liquid chromatography-high resolution mass spectrometry[J]. Food and Fermentation Industries, 2022
, 48(1)
: 253
-260
.
DOI: 10.13995/j.cnki.11-1802/ts.028473
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