Tuber sinense is one of the most important truffles in commercial, Panzhihua and Huidong are two main producing regions of T. sinense. The truffles from Panzhihua and Huidong were analyzed by GC-MS. The results of PCA indicated that the composition difference between the two truffles were not significant. The truffles, from both regions, contained 83 of effective compounds, 15 of amino acids (8 of which are essential ones), 10 of saccharides, and 22 of organic acids. The results of PLS-DA indicated a significant difference in the content of galactinol, uridine, octadecadien, 9-(E)-octadece, and arabinonic acid were detected. These results mitigated the limitations of the traditional ITS identification method that cannot distinguish the producing area of Tuber sinense.
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