Food and Fermentation Industries >

2022 , Vol. 48 >Issue 2: 8 - 14

DOI: https://doi.org/10.13995/j.cnki.11-1802/ts.027307

Recombinant Corynebacterium glutamicum whole cell catalyzes one-step synthesis of high fructose syrup

  • HABIMANA ,
  • QIAO Zhina ,
  • XU Meijuan ,
  • YANG Taowei ,
  • ZHANG Xian ,
  • SHAO Minglong ,
  • RAO Zhiming
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  • (School of Biotechnology, Jiangnan University, Wuxi 214122, China)

Received date: 2021-03-10

  Revised date: 2021-05-05

  Online published: 2022-02-28

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Abstract

High fructose syrup is a sweetener that can replace sucrose, which is widely applied in the food and beverage industries. In this study, the codon-optimized glucose isomerase from Actinoplanes missouriensis CICIM B0118 (A) was expressed heterologously in the food-safe strain Corynebacterium glutamicum 13032, and the recombinant C. glutamicum 13032/pXMJ19-xylA was constructed. The whole cell conversion system of D-glucose isomerization to the biosynthesis of D-fructose was optimized. The results showed that the yield of D-fructose reached 103.32 g/L when the continuous conversion was performed at 70 ℃ and 220 r/min for 18 h, with 40 g/L bacterial cells, 180 g/L D-glucose, 10 mmol/L Mg2+ , and 1 mmol/L Co2+ . The conversion rate was 57.4%, and the safe one-step biosynthesis of high fructose syrup was achieved. This research provided an experimental and theoretical basis for the industrialized synthesis of high fructose syrup, and had important reference value.

Key words： glucose isomerase; isomerization; whole cell catalysis; Corynebacterium glutamicum; high fructose syrup

Cite this article

HABIMANA , QIAO Zhina , XU Meijuan , YANG Taowei , ZHANG Xian , SHAO Minglong , RAO Zhiming . Recombinant Corynebacterium glutamicum whole cell catalyzes one-step synthesis of high fructose syrup[J]. Food and Fermentation Industries, 2022 , 48(2) : 8 -14 . DOI: 10.13995/j.cnki.11-1802/ts.027307

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