Turanose has the characteristics of low calorie and non-caries properties, so it has a wide application prospect in food industry. Compared with amylosucrase, the Caulobacter crescentus sucrose hydrolase mutant S271A (CcSH S271A) produced fewer malto-oligosaccharide by-products, which can replace amylosucrase for the preparation of turanose. In this study, the CcSH S271A was recombinantly expressed in Bacillus subtilis, the enzymatic properties of recombinant CcSH S271A were identified and the process of preparing turanose by recombinant CcSH S271A was optimized. The results showed that the optimal reaction temperature and reaction pH of recombinant CcSH S271A were 45 ℃ and 8.0, respectively. The recombinant CcSH S271A displayed excellent thermal stability at 30 ℃ with a half-life of 312 h. The yield of turanose was 66.3% with 2 mol/L sucrose as the substrate, when the amount of enzyme was 60 U/g sucrose, at pH 8.0 and 30 ℃ for 48 h. It is the highest level of preparing turanose using sucrose as a single substrate, which lays a solid foundation for the preparation of turanose.
XING Chenchen
,
WANG Lei
,
GUO Zhiyong
,
ZHANG Kang
,
TAO Xiumei
,
WU Jing
. Recombinant expression of Caulobacter crescentus sucrose hydrolase mutant S271A and its conversion of sucrose to turanose[J]. Food and Fermentation Industries, 2022
, 48(2)
: 20
-25
.
DOI: 10.13995/j.cnki.11-1802/ts.027753
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