The purpose of this study was to investigate the in vitro degradation of 3-methylindole by lactic acid bacteria(LAB) isolated from human intestine. MRS medium were used to screen LAB from the intestine of healthy children (aged 3 to 8 years), and then, total bacterial DNA was extracted, 16S rRNA sequence was amplified and the phylogenetic tree was constructed. LABs were cultured under stress in inorganic salt medium using 3-methylindole as sole carbon source and nitrogen source. The absorbance of LAB in high concentration skatole environment was measured by UV photometer. Then the degradation of 3-methylindole by LAB was determined by dynamic determination of 3-methylindole residue by GC-MS. The results showed that eight strains were all LAB, among which LAB1 belonged to Lactobacillus casei, LAB2 belonged to Weissella paramesenteroides, LAB3, LAB5, LAB6, LAB7, LAB8 belonged to Leuconostoc mesenteroides, and LAB4 belonged to Lactococcus garvieae. The eight strains grew slowly in the inorganic salt medium supplemented with 3-methylindole, but had strong ability to degrade 3-methylindole. The in vitro degradation rates of LAB1-LAB8 to 3-methylindole were 62.21%, 60.45%, 52.50%, 62.19%, 61.91%, 61.94%, 55.34% and 58.73%, respectively at 168 h, and the degradation rate of LAB1 (Lactobacillus casei) was the highest. It was proved that the LAB from children's intestines could degrade 3-methylindole. The results of our study provide a new microbial resource for degrading enterotoxin, and also provide a feasible theoretical basis for expanding the application of LAB in the future.
ZHUANG Weiwei
,
ZHU Yaqin
,
ZENG Xianchun
. Isolation and identification of lactic acid bacteria from intestinal tract of children and its function in the degradation of 3-methylindole[J]. Food and Fermentation Industries, 2022
, 48(2)
: 144
-149
.
DOI: 10.13995/j.cnki.11-1802/ts.027719
[1] GONZALEZ L M, MOESER A J, BLIKSLAGER A T.Porcine models of digestive disease:The future of large animal translational research[J].Translational Research, 2015, 166(1):12-27.
[2] BHARUCHA A E, PEMBERTON J H, LOCKE G R Ⅲ.American gastroenterological association technical review on constipation[J].Gastroenterology, 2013, 144(1):218-238.
[3] 陈辰, 房静远.饮食因素影响结直肠癌发生的研究进展[J].中华医学杂志, 2014, 94(26):2 069-2 072.
CHEN C, FANG J Y.Advances in the study of dietary factors affecting colorectal carcinogenesis[J].National Medical Journal of China, 2014, 94(26):2 069-2 072.
[4] BELGHITI D K, SCORSONE E, DE SANOIT J, et al.Simultaneous detection of indole and 3-methylindole using boron-doped diamond electrodes[J].Physica Status Solidi, 2016, 213(10):2 662-2 671.
[5] SIVAN A, CORRALES L, HUBERT N, et al.Commensal Bifidobacterium promotes antitumor immunity and facilitates anti-PD-L1 efficacy[J].Science, 2015, 350(6 264):1 084-1 089.
[6] HUANG Y, WANG J F, CHENG Y, et al.The hypocholesterolaemic effects of Lactobacillus acidophilus American type culture collection 4356 in rats are mediated by the down-regulation of Niemann-Pick C1-Like 1[J].The British Journal of Nutrition, 2010, 104(6):807-812.
[7] 田丰松, 王军, 杨连玉, 等.不同乳酸菌分离株抗菌物质及其抗菌活性的体外检测及鉴定[J].中国预防兽医学报, 2016, 38(7):558-561.
TIAN F S, WANG J, YANG L Y, et al.In vitro analysis of substance with antibacterial activity of lactic acid bacteria[J].Chinese Journal of Preventive Veterinary Medicine, 2016, 38(7):558-561.
[8] BEN SALAH-ABBÈS J,ABBÈS S, JEBALI R, et al.Potential preventive role of lactic acid bacteria against AFM1 immunotoxicity and genotoxicity in mice[J].Journal of Immunotoxicology, 2015, 12(2):107-114.
[9] ABRUNHOSA L, INÊS A, RODRIGUES A I, et al.Biodegradation of ochratoxin A by Pediococcus parvulus isolated from Douro wines[J].International Journal of Food Microbiology, 2014, 188:45-52.
[10] ARORA P K,SHARMA A, BAE H H.Microbial degradation of indole and its derivatives[J].Journal of Chemistry, 2015.DOI:10.1155/2015/129159.
[11] 朱雅琴, 黄煦杰, 江岩, 等.骆驼瘤胃乳酸菌的分离、鉴定及其降解吲哚的功能研究[J].食品工业科技, 2020, 41(11):134-139;145.
ZHU Y Q, HUANG X J, JIANG Y, et al.Isolation and identification of lactic acid bacteria from rumen of camel and its function in degradation of indole[J].Science and Technology of Food Industry, 2020, 41(11):134-139;145.
[12] MA Q, QU H, MENG N, et al.Biodegradation of skatole by Burkholderia sp.IDO3 and its successful bioaugmentation in activated sludge systems[J].Environmental Research, 2020, 182:109123.
[13] SHARMA N, DOERNER K C, ALOK P C, et al.Skatole remediation potential of Rhodopseudomonas palustris WKU-KDNS3 isolated from an animal waste lagoon[J].Letters in Applied Microbiology, 2015, 60(3):298-306.
[14] YIN B O, HUANG L M, GU J D.Biodegradation of 1-methylindole and 3-methylindole by mangrove sediment enrichment cultures and a pure culture of an isolated Pseudomonas aeruginosags[J].Water, Air, and Soil Pollution, 2006, 176(1-4):185-199.
[15] TESSO T A, ZHENG A J, CAI H Y, et al.Isolation and characterization of two Acinetobacter species able to degrade 3-methylindole[J].PLoS One, 2019, 14(1):e0211275.
[16] GU J D, FAN Y Z, SHI H C.Relationship between structures of substituted indolic compounds and their degradation by marine anaerobic microorganisms[J].Marine Pollution Bulletin, 2002, 45(1):379-384.
[17] BOROWSKI S, MATUSIAK K, POWAŁOWSKI S, et al.A novel microbial-mineral preparation for the removal of offensive odors from poultry manure[J].International Biodeterioration & Biodegradation, 2017, 119:299-308.
[18] 曾正清. 蚯蚓和乳酸菌(L4)对猪粪尿臭气浓度和免疫机能的影响及其机理研究[D].北京:中国农业大学, 2003.
ZENG Z Q.Effect of earthworms and lactic acid bacteria (L4) on odor concentration and immune function of pig feces and urine and their mechanism[D].Beijing:China Agricultural University, 2003.
[19] MENG X, HE Z F, LI H J, et al.Removal of 3-methylindole by lactic acid bacteria in vitro[J].Experimental & Therapeutic Medicine, 2013, 6(4):983-988.
