Food and Fermentation Industries >

2022 , Vol. 48 >Issue 2: 156 - 162

DOI: https://doi.org/10.13995/j.cnki.11-1802/ts.027445

Intestinal probiotic effect of Akkermansia muciniphila ATCC BAA-835 in vitro

  • WU Yanli ,
  • LIU Peng ,
  • SU Yongxin ,
  • LI Heng ,
  • GENG Yan ,
  • REN Yilin ,
  • XU Hongyu ,
  • XU Zhenghong ,
  • SHI Jinsong
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  • 1(School of Life Science and Health Engineering, Jiangnan University, Wuxi 214122, China)
    2(Key Laboratory of Carbohydrate Chemistry and Biotechnology, Ministry of Education, Wuxi 214122, China)
    3(National Engineering Research Center for Cereal Fermentation and Food Biomanufacturing, Wuxi 214122, China)
    4(School of Biotechnology, Jiangnan University, Wuxi 214122, China)

Received date: 2021-03-24

  Revised date: 2021-04-16

  Online published: 2022-02-28

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Abstract

Akkermansia muciniphila (Akk) possesses superior intestinal probiotic effect which is closely related to the host's physiological conditions, including intestinal, metabolic and neurological health. In this study, the intestinal probiotic effect of an Akk model strain (ATCC BAA-835) was evaluated using anaerobic cultivation method in vitro. The probiotic abilities of Akk were investigated, including the tolerance to simulated gastrointestinal environment, adhesion to Caco-2 cells, production of short-chain fatty acids and formation of biofilms utilizing oligosaccharides. The results showed that this Akk stain performed good tolerance to artificial gastrointestinal fluids and bile salts. It could adhere to the microvilli tip of differentiated Caco-2 cells. This Akk strain grew well in the medium with various oligosaccharides. It could quickly consume reducing sugars within 12 h and produce short-chain fatty acids, of which the output of acetic acid was the highest, followed by butyric acid. Akk possessed weak biofilm-forming ability, which could be improved significantly with the addition of oligosaccharides. And Akk could form biofilms at a medium level when the concentration of chito-oligosaccharides was 250 μg/mL. This study could provide further understanding for the microbiological basis of Akk's intestinal probiotic effect with assessments in vitro including pure microbial culture and cell models.

Key words： Akkermansia muciniphila; pure culture; assessment in vitro; oligosaccharides; probiotic effect

Cite this article

WU Yanli , LIU Peng , SU Yongxin , LI Heng , GENG Yan , REN Yilin , XU Hongyu , XU Zhenghong , SHI Jinsong . Intestinal probiotic effect of Akkermansia muciniphila ATCC BAA-835 in vitro[J]. Food and Fermentation Industries, 2022 , 48(2) : 156 -162 . DOI: 10.13995/j.cnki.11-1802/ts.027445

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