Food and Fermentation Industries >

2022 , Vol. 48 >Issue 3: 38 - 43

DOI: https://doi.org/10.13995/j.cnki.11-1802/ts.028306

Effect of prokaryotic expression conditions of Zea mays flavanone-3-hydroxylase on production of aromadendrin

  • HUANG Xu ,
  • WANG Zhen ,
  • LIU Juan ,
  • XIAO Feiyao ,
  • TIAN Miaomiao ,
  • GUO Jiajing ,
  • SHAN Yang
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  • 1(Longping Branch Graduate School,Hunan University,Changsha 410082,China)
    2(Institute of Agricultural Product Processing,Hunan Academy of Agricultural Sciences/Hunan Province Key Lab of Fruits & Vegetables Storage,Processing,Quality and Safety/Hunan Province International Joint Lab on Fruits & Vegetables Processing,Quality and Safety,Changsha 410125,China)

Received date: 2021-06-08

  Revised date: 2021-07-13

  Online published: 2022-03-04

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Abstract

Aromadendrin is an important intermediate compound in flavonoid metabolism pathway. Flavanone-3-hydroxylase (F3H) catalyzes hydroxyl grafting of naringin at C3 site. Microbial method was used to achieve the green development of aromadendrin. ZmF3H protein was expressed by Escherichia coli containing Zmf3h from Zea mays. Fermentation experiments confirmed that ZmF3H was soluble in intracellular and successfully catalyzed the production of aromadendrin. The results showed that the production of aromadendrin was significantly increased when the final IPTG concentration was 0.6 mmol/L, the induction temperature was 23 ℃, the induction time was 8 h, and the cell density was between 0.8 to 1.0. The production of aromadendrin was (32.27±1.98) mg/L after optimization which was 60.39% higher than that of initial conditions.

Key words： aromadendrin; flavanone-3-hydroxylase; prokaryotic expression; expression optimization

Cite this article

HUANG Xu , WANG Zhen , LIU Juan , XIAO Feiyao , TIAN Miaomiao , GUO Jiajing , SHAN Yang . Effect of prokaryotic expression conditions of Zea mays flavanone-3-hydroxylase on production of aromadendrin[J]. Food and Fermentation Industries, 2022 , 48(3) : 38 -43 . DOI: 10.13995/j.cnki.11-1802/ts.028306

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