Food and Fermentation Industries >

2022 , Vol. 48 >Issue 3: 44 - 49

DOI: https://doi.org/10.13995/j.cnki.11-1802/ts.028423

Breeding of L-tryptophan high-yield strain by ARTP mutagenesis

  • ZHOU Xubo ,
  • CHEN Yuqi ,
  • DING Ding ,
  • LIU Shuai ,
  • WANG Jian
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  • (Jiangsu AC Biotech Co.Ltd.,Wuxi 214000,China)

Received date: 2021-06-21

  Revised date: 2021-07-28

  Online published: 2022-03-04

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Abstract

To improve the efficiency of L-tryptophan production, atmospheric and room temperature plasma (ARTP) mutagenesis combined with analogue resistance screening was used to obtain high-yield L-tryptophan production strain. Escherichia coli AC-1042 was mutated by ARTP mutagenesis, and the L-tryptophan genetic stable high-yield strain was selected by 5-methyltryptophan (5-MT) and p-fluorophenylalanine (PFP) resistance. After multiple ARTP mutagenesis processing and improving the analogue resistance screening, a genetic stable high-yield tryptophan strain ACTRP104 was obtained. The tryptophan mutant ACTRP104 produced 61.65 g/L L-tryptophan at a yield of 20.64% of glucose in the 30 L fermentation tank at 44 h, which was increased by 20.69% and 17.81% compared with the original strain, respectively. These results suggested that it was effective to improve the production capacity of tryptophan strains by combining of ARTP mutagenesis with analogue resistance screening. The tryptophan high-yield mutant ACTRP104 has a great potential for industrial application.

Key words： atmospheric and room temperature plasma (ARTP); L-tryptophan; mutation breeding; resistance screening; high-yield mutant

Cite this article

ZHOU Xubo , CHEN Yuqi , DING Ding , LIU Shuai , WANG Jian . Breeding of L-tryptophan high-yield strain by ARTP mutagenesis[J]. Food and Fermentation Industries, 2022 , 48(3) : 44 -49 . DOI: 10.13995/j.cnki.11-1802/ts.028423

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