To explore the relationship between the dose of compound probiotics and intestinal microflora, growth performance, metabolism of experimental animal, 6-week-old BALB/C mice were intragastric administration with 108 (low dose), 109 (medium dose) and 1010 CFU/mL (high dose) compound probiotics every day for 6 weeks. The weight gain, serum biochemical indexes, organ structure and intestinal microflora were assessed. The results showed that the weight gain and feed utilization rate of mice in medium and low dose group were significantly higher than that of control, and the metabolic function of intestinal microorganisms in mice was enhanced. There was no significant difference of serum total protein and albumin. However, changes in the structure of intestine, liver and kidney in high dose groups were observed. The activities of aspartate aminotransferase (AST) and alanine aminotransferase (ALT) were (69.8±5.2) and (52.7±3.1) in high dose group, which was significantly higher than those in other groups. There was slight fibrosis around liver tissue and slight atrophy of glomerulus, which indicated that damage of liver and kidney cells were induced by high dose compound probiotics intragastric administration. The intestinal flora structure analysis suggested that the Firmicutes decreased, the Bacteroides and Proteus increased, and the metabolic function abundance decreased in high dose group. Therefore, supplement probiotics properly can promote health of body. Excessive doses may pose a risk to the health of human and animal.
[1] LI J H,JIA H J,CAI X H,et al.An integrated catalog of reference genes in the human gut microbiome[J].Nature Biotechnology,2014,32(8):834-841.
[2] WLODARSKA M,FINLAY B B.Host immune response to antibiotic perturbation of the microbiota[J].Mucosal Immunology,2010,3(2):100-103.
[3] RAO S,HU S,MCHUGH L,et al.Toward a live microbial microbicide for HIV:Commensal bacteria secreting an HIV fusion inhibitor peptide[J].PNAS,2005,102(34):11 993-11 998.
[4] 林显华, 孙合美,谷巍.枯草芽胞杆菌B7对肉鸡生长性能及免疫性能的影响[J].中国畜牧兽医,2013,40(2):91-95.
LIN X H,SUN H M,GU W.Effects of Bacillus subtilis on growth performance and immune function of broilers[J].China Animal Husbandry & Veterinary Medicine,2013,40(2):91-95.
[5] WEI X B,LIAO X D,CAI J,et al.Effects of Bacillus amyloliquefaciens LFB112 in the diet on growth of broilers and on the quality and fatty acid composition of broiler meat[J].Animal Production Science,2017,57(9):1 899-1 905.
[6] WANG H S,NI X Q,QING X D,et al.Live probiotic Lactobacillus johnsonii BS15 promotes growth performance and lowers fat deposition by improving lipid metabolism,intestinal development,and gut microflora in broilers[J].Frontiers in Microbiology,2017,8:1073.
[7] RAO S S C,REHMAN A,YU S,et al.Brain fogginess,gas and bloating:A link between SIBO,probiotics and metabolic acidosis[J].Clinical and Translational Gastroenterology,2018,9(6):162.
[8] ZMORA N,ZILBERMAN-SCHAPIRA G,SUEZ J,et al.Personalized gut mucosal colonization resistance to empiric probiotics is associated with unique host and microbiome features[J].Cell,2018,174(6):1 388-1 405.
[9] WANG J,ZHANG H,CHEN X,et al.Selection of potential probiotic Lactobacilli for cholesterol-lowering properties and their effect on cholesterol metabolism in rats fed a high-lipid diet[J].Journal of Dairy Science,2012,95(4):1 645-1 654.
[10] 曾东,李涛,倪学勤,等.乳酸杆菌和囊素三肽对肉鸡生长性能、屠宰性能、肉质和血清生化指标的影响[J].动物营养学报,2014,26(5):1 320-1 326.
ZENG D,LI T,NI X Q,et al.Effects of Lactobacilli and bursin on growth performance,slaughter performance,meat quality and serum biochemical parameters of broiler[J].Chinese Journal of Animal Nutrition,2014,26(5):1 320-1 326.
[11] 郭元晟,闫素梅,史彬林,等.发酵乳酸杆菌对肉鸡小肠绒毛形态的影响[J].动物营养学报,2011,23(7):1 194-1 200.
GUO Y S,YAN S M,SHI B L,et al.Effects of Lactobacillus fermentum supplementation on the small intestinal villus structure of broilers[J].Chinese Journal of Animal Nutrition,2011,23(7):1 194-1 200.
[12] WIEST R,LAWSON M,GEUKING M.Pathological bacterial translocation in liver cirrhosis[J].Journal of Hepatology,2014,60(1):197-209.
[13] CANI P D,AMAR J,IGLESIAS M A,et al.Metabolic endotoxemia initiates obesity and insulin resistance[J].Diabetes,2007,56(7):1 761-1 772.
[14] FELIZARDO R J F,CASTOLDI A,ANDRADE-OLIVEIRA V,et al.The microbiota and chronic kidney diseases:A double-edged sword[J].Clinical & Translational Immunology,2016,5(6):e86.
[15] RAMEZANI A,MASSY Z A,MEIJERS B,et al.Role of the gut microbiome in uremia:A potential therapeutic target[J].American Journal of Kidney Diseases,2016,67(3):483-498.
[16] VAZIRI N D.Effect of synbiotic therapy on gut-derived uremic toxins and the intestinal microbiome in patients with CKD[J].Clinical Journal of the American Society of Nephrology,2016,11(2):199-201.
[17] BAJAJ J S,BETRAPALLY N S,HYLEMON P B,et al.Salivary microbiota reflects changes in gut microbiota in cirrhosis with hepatic encephalopathy[J].Hepatology,2015,62(4):1 260-1 271.
[18] FERNÁNDEZ J,ACEVEDO J,WIEST R,et al.Bacterial and fungal infections in acute-on-chronic liver failure:Prevalence,characteristics and impact on prognosis[J].Gut,2018,67(10):1 870-1 880.
[19] LLORENTE C,JEPSEN P,INAMINE T,et al.Gastric acid suppression promotes alcoholic liver disease by inducing overgrowth of intestinal Enterococcus[J].Nature Communications,2017,8(1):837.
[20] WONG J,PICENO Y M,DESANTIS T Z,et al.Expansion of urease-and uricase-containing,indole-and p-cresol-forming and contraction of short-chain fatty acid-producing intestinal microbiota in ESRD[J].American Journal of Nephrology,2014,39(3):230-237.
[21] THOMAS C M,HONG T,VAN PIJKEREN J P,et al.Histamine derived from probiotic Lactobacillus reuteri suppresses TNF via modulation of PKA and ERK signaling[J].PLoS One,2012,7(2):e31951.
[22] GAO J,XU K,LIU H N,et al.Impact of the gut microbiota on intestinal immunity mediated by tryptophan metabolism[J].Frontiers in Cellular and Infection Microbiology,2018,8:13.
[23] EVENEPOEL P,POESEN R,MEIJERS B.The gut–kidney axis[J].Pediatric Nephrology,2017,32(11):2 005-2 014.
[24] GILL T,BROOKS S R,ROSENBAUM J T,et al.Novel inter-omic analysis reveals relationships between diverse gut microbiota and host immune dysregulation in HLA-B27-induced experimental spondyloarthritis[J].Arthritis & Rheumatology,2019,71(11):1 849-1 857.
[25] SCHNEIDER K M,ELFERS C,GHALLAB A,et al.Intestinal dysbiosis amplifies acetaminophen-Induced acute liver injury[J].Cellular and Molecular Gastroenterology and Hepatology,2021,11(4):909-933.
[26] KOLODZIEJCZYK A A,FEDERICI S,ZMORA N,et al.Acute liver failure is regulated by MYC-and microbiome-dependent programs[J].Nature Medicine,2020,26(12):1 899-1911
[27] TRANAH T H,EDWARDS L A,SCHNABL B,et al.Targeting the gut-liver-immune axis to treat cirrhosis[J].Gut,2021,70(5):982-994.
