In order to get a rapid, convenient and accurate method for the determination of choline in food, this paper explored the immobilization method of enzyme on photosensitive electrode for the determination of choline content. Choline oxidase was immobilized by chitosan embedding and glutaraldehyde crosslinking. The effects of chitosan concentration, glutaraldehyde concentration and immobilization time on the activity of choline oxidase were studied by single factor experiment and response surface analysis. The immobilization conditions of choline oxidase were optimized. When mass fraction of chitosan was 1%, mass fraction of glutaraldehyde was 0.55% and immobilization time was 1 h, the activity of immobilized choline oxidase was the highest, reaching 17.2 U. Scanning electron microscopy showed that choline oxidase was successfully immobilized on the surface of the photosensitive electrode. In addition, the activity of choline oxidase immobilized on the photosensitive electrode remained at 83% after 10 times of reuse and 86% after 20 days of storage. The choline oxidase immobilized on the photosensitive electrode had good reusability and storage stability. The method preliminarily explored the development process of the photosensitive electrode for the determination of choline in food, which lays a theoretical foundation for the rapid determination of choline in food.
SONG Yang
,
WANG Weining
,
YAO Jing
,
WANG Ruiying
,
LUO Shunian
,
WANG Liqi
. Immobilization of choline oxidase on electrode for choline determination[J]. Food and Fermentation Industries, 2022
, 48(4)
: 56
-61
.
DOI: 10.13995/j.cnki.11-1802/ts.027675
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