Food and Fermentation Industries >

2022 , Vol. 48 >Issue 5: 1 - 7

DOI: https://doi.org/10.13995/j.cnki.11-1802/ts.029337

Preparation and characterization of cross-linked enzyme aggregates of phospholipase D

  • CHEN Ning ,
  • YAN Wenxing ,
  • LU Fuping ,
  • LIU Fufeng
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  • (Key Laboratory of Industrial Fermentation Microbiology,Ministry of Education,Tianjin Key Laboratory of Industrial Microbiology,National Engineering Laboratory for Industrial Enzymes,College of Biotechnology,Tianjin University of Science & Technology,Tianjin 300457,China)

Online published: 2022-04-06

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Abstract

Phospholipase D can catalyze the hydrolysis of phosphodiester bond of phospholipids, and the conversion of phospholipid acyl reaction. Phospholipase D is widely used in chemical and pharmaceutical industries. In this study, a novel cross-linked phospholipase D aggregates (PLD-CLEAs) was developed. Using the recovery rate of enzyme activity as the main indicator, the optimal preparation conditions of PLD-CLEAs were determined as follows: the precipitant was ammonium sulfate with 60% saturation, the crosslinking agent was glutaraldehyde, and its mass fraction was 0.125%, and the crosslinking time was 1.5 h. The enzyme activity recovery of PLD-CLEAs prepared under the optimum conditions was 118.8%. Compared with free PLD, the optimal pH of PLD-CLEAs shifted towards neutral direction (from 8.0 to 7.0). And PLD-CLEAs were maintained a relatively high enzyme activity in wide temperature and pH ranges. Moreover, the thermal stability of PLD-CLEAs was improved obviously at high temperature (50 ℃). In addition, PLD-CLEAs still had 50.4% of initial enzyme activity after 10 repeated uses. The above research results showed that PLD-CLEAs had superior thermal and pH stabilities and reusability in comparison with the free ones, thus has a good prospect of industrial application.

Key words： phospholipase D; carrier-free immobilization; cross-linked enzyme aggregates; enzymatic characteristics; reusability

Cite this article

CHEN Ning , YAN Wenxing , LU Fuping , LIU Fufeng . Preparation and characterization of cross-linked enzyme aggregates of phospholipase D[J]. Food and Fermentation Industries, 2022 , 48(5) : 1 -7 . DOI: 10.13995/j.cnki.11-1802/ts.029337

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