Selenium-enriched yeast is one of the important sources of selenium supplement due to its high content of organic selenium, excellent physiological activity, and safety. In this study, glucose and fructose were employed respectively as carbon sources to investigate the effects of fructose on selenium metabolism and gene expression in Hansenula polymorpha. The selenomethionine levels in H. polymorpha were detected using HPLC-ICP-MS, and transcriptome profiles were investigated using the next-generation sequencing technology. Compared with the glucose fermentation group, the content of selenomethionine in H. polymorpha was increased when 80g/L fructose was added; however, the dry cell weight was decreased. A total of 221 differential expression genes (DEGs) were identified between the fructose-fermentation group and the glucose-fermentation group, among which 80 genes were up-regulated, and 141 genes were down-regulated. Based on the Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment, these DEGs were mainly enriched in fructose and mannose metabolism, glycolysis, pentose phosphate pathway, tricarboxylic acid cycle, glutathione metabolism, as well as cysteine, methionine, and selenium complex metabolic pathways. This study provides a reference for further analysis of the effect of fructose on selenium metabolism in H. polymorpha and the optimization of fermentation conditions by selenium-enriched yeast.
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