Food and Fermentation Industries >

2022 , Vol. 48 >Issue 5: 35 - 40;46

DOI: https://doi.org/10.13995/j.cnki.11-1802/ts.028183

Identification of naphthoquinone-responsive gene in Monascus strain for improvement of Monascus pigments yield

  • FAN Fei ,
  • DUAN Yali ,
  • LIU Yapeng ,
  • YU Peilin ,
  • LEI Ming ,
  • CHEN Shaoyun ,
  • LI Mu
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  • 1(College of Food Science and Technology,Huazhong Agricultural University,Wuhan 430070,China)
    2(School of Life Sciences,Zhejiang Chinese Medical University,Hangzhou 310000,China)

Online published: 2022-04-06

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Abstract

Monascus pigments, which are produced by Monascus strains, exhibit bright colors. Monascus pigment is one of the natural food pigments with high sales in China. In our previous work, it was found that exogenous naphthoquinone improved the yield of Monascus pigments. However, the genes responsible for naphthoquinone response in Monascus strains are still unknow. Thus, it is hard to facilitate the practical application. In this study, it was verified that Monascus pigments yield of M. ruber M7 was promoted by the addition of exogenous naphthoquinone. Secondly, two naphthoquinone-responsive gene candidates, laeA and mga2, were revealed by a combination of transcriptomics and artificial intelligence analysis. Thirdly, mga2 gene (G protein β subunit) was identified as a naphthoquinone-responsive gene in M. ruber M7 by the direct feeding assay. Lastly, the strain M7:PtrpC-mga2 displayed an increased Monascus pigments yield, 74% higher than that of wild type strain under the plumbagin induction condition. For the first time, this study elucidated that mga2 gene is the naphthoquinone-responsive gene in Monascus spp. This discovery was successfully employed to improve Monascus pigments yield. Furthermore, our research could be used as reference for elucidating the similar induction mechanism in other filamentous fungi.

Key words： Monascus spp.; Monascus pigments; artificial intelligence; naphthoquinone; responsive gene

Cite this article

FAN Fei , DUAN Yali , LIU Yapeng , YU Peilin , LEI Ming , CHEN Shaoyun , LI Mu . Identification of naphthoquinone-responsive gene in Monascus strain for improvement of Monascus pigments yield[J]. Food and Fermentation Industries, 2022 , 48(5) : 35 -40;46 . DOI: 10.13995/j.cnki.11-1802/ts.028183

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