Food and Fermentation Industries >

2022 , Vol. 48 >Issue 5: 106 - 110

DOI: https://doi.org/10.13995/j.cnki.11-1802/ts.028252

Optimization of cordycepin production from Cordyceps militaris

  • TANG Chenglun ,
  • WANG Songtao ,
  • ZHAN Nan ,
  • ZOU Yanan ,
  • CHEN Yong ,
  • LIU Qingguo ,
  • LIU Miao
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  • 1(Nanjing Hi-tech Biological Technology Research Institute Co.Ltd.,Nanjing 210032,China)
    2(Luzhou Laojiao Co.Ltd.,Luzhou 646000,China)
    3(College of Biotechnology and Pharmaceutical Engineering Nanjing University of Technology,Nanjing 210032,China)

Online published: 2022-04-06

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Abstract

In order to determine the amplification technology for cordycepin, the effects of cultivation quality and height-diameter ratio of fermentation on the synthesis of cordycepin were investigated. The results showed that the concentration of cordycepin reached 6 g/L when the optimal conditions of seed cultivation were as follows: the rotation speed of 250 r/min, the inoculum size of 15%, and the subculture times of two. In addition, the optimal height-diameter ratio of liquid loading was 2 cm/30 cm. Under these conditions, a 120 L multi-layer reactor was used to verify the fermentation process. After 25 days of fermentation, the concentration of cordycepin was 5.32 g/L and the conversion rate was 57%, which was consistent with the results of small scale test. The experimental results showed that the process scaling up is feasible, which provides a reference for further industrialization.

Key words： Cordyceps militaris; cordycepin; process amplification; multilayer reactor; height/diameter ratio

Cite this article

TANG Chenglun , WANG Songtao , ZHAN Nan , ZOU Yanan , CHEN Yong , LIU Qingguo , LIU Miao . Optimization of cordycepin production from Cordyceps militaris[J]. Food and Fermentation Industries, 2022 , 48(5) : 106 -110 . DOI: 10.13995/j.cnki.11-1802/ts.028252

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