Microfluidic technology can integrate basic units such as sample preparation, separation, and detection on a chip which is only a few square centimeters. It can be combined with molecular detection technology to meet the requirements of rapid on-site testing of food-borne pathogens. Based on the loop-mediated isothermal amplification technology, a centrifugal microfluidic chip for the rapid detection of food-borne pathogens was designed and manufactured. The chip included a sample reservoir, aliquoting chambers, reaction chambers, an injection hole, a vent, capillary valves and other structures. The centrifugal microfluidic nucleic acid isothermal amplification device realized fluid control, isothermal amplification and visual detection on the microfluidic chip, and could detect three food-borne pathogens in five samples at a time. The results showed that E. coli, Salmonella and Listeria monocytogenes could be detected with the loop-mediated isothermal amplification (LAMP)-based centrifugal microfluidic method within 60 minutes, and the results had good specificity and sensitivity. The lowest detection limit was 103copies/μL. Microfluidic technology is used for nucleic acid extraction, isothermal amplification and detection of food-borne pathogens. It has the advantages of integration, ease of carrying and rapid analysis, showing the potential of microfluidic technology in the rapid detection of food safety.
YAO Yanlu
,
CAO Ning
,
ZHOU Xinli
. The detection of three pathogenic bacteria by centrifugal microfluidic chip based on LAMP methodology[J]. Food and Fermentation Industries, 2022
, 48(5)
: 255
-261
.
DOI: 10.13995/j.cnki.11-1802/ts.029423
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