Food and Fermentation Industries >

2022 , Vol. 48 >Issue 5: 262 - 267

DOI: https://doi.org/10.13995/j.cnki.11-1802/ts.029702

High-throughput detection of organophosphorus pesticide residues in fresh meat by QuEChERS-GC-MS/MS

  • WANG Shasha ,
  • SHEN Xixi ,
  • LI Manman ,
  • SHEN Juan ,
  • TANG Changbo ,
  • WANG Wei
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  • 1(College of Food Science and Technology,Nanjing Agricultural University,Nanjing 210095,China)
    2(National Center of Meat Quality and Safety Control,Nanjing Agricultural University,Nanjing 210095,China)
    3(Supervision,Inspection and Testing Center for Quality of Meat-products(Nanjing),Ministry of Agriculture and Rural Affairs,Nanjing Agricultural University,Nanjing 210095,China)

Online published: 2022-04-06

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Abstract

This study aimed to establish a rapid and effective QuEChERS-GC-MS/MS detection method for 18 organophosphorus pesticide residues in fresh meat. The sample was extracted with saturated n-hexane in acetonitrile and purified by dispersion solid-phase extraction with MgSO4, CH3COONa, N-propyl ethylenediamine (PSA) and octadecyl bonded silica gel (C18). Then DB-1701 capillary column was used for the separation, and GC-MS/MS was used for the detection. Organophosphorus pesticides showed a good linear relationship in the range of 1-100 μg/kg, and the square of correlation coefficient (R2) were all greater than 0.990 0. The detection limit was 0.02-0.74 μg/kg and the quantification limit was 0.08-2.48 μg/kg. Meanwhile, the recovery rate reached 76%-106% and the relative standard deviation was 0.50%-7.9%. The QuEChERS-GC-MS/MS method established in this study can simultaneously detect 18 organophosphorus pesticides. It is simple, fast, sensitive and accurate, which can meet the high throughput and rapidity of organophosphorus pesticide residues in fresh livestock and poultry meat.

Key words： organophosphorus pesticides; fresh meat; QuEChERS; GC-MS/MS; detection

Cite this article

WANG Shasha , SHEN Xixi , LI Manman , SHEN Juan , TANG Changbo , WANG Wei . High-throughput detection of organophosphorus pesticide residues in fresh meat by QuEChERS-GC-MS/MS[J]. Food and Fermentation Industries, 2022 , 48(5) : 262 -267 . DOI: 10.13995/j.cnki.11-1802/ts.029702

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